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Directed evolution

Heuristic algorithms in evolutionary computation and modular organization of biological macromolecules: Applications to in vitro evolution

Alexander V. Spirov, Ekaterina M. Myasnikova

Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology

Roberto Laos, Steven Benner

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems

Ce Shi, Michael P. Killoran, [ ... ], Thomas A. Kirkland

Improved yellow-green split fluorescent proteins for protein labeling and signal amplification

Shuqin Zhou, Siyu Feng, David Brown, Bo Huang

SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

Derek Cerchione, Katherine Loveluck, [ ... ], Barrett E. Steinberg

The scale-free nature of protein sequence space

Patrick C. F. Buchholz, Catharina Zeil, Jürgen Pleiss

Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae

Chenlu Zhang, Ligia Acosta-Sampson, Vivian Yaci Yu, Jamie H. D. Cate

Directed evolution provides insight into conformational substrate sampling by SrtA

Muna Suliman, Vishaka Santosh, [ ... ], William A. Barton

Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis

Wenjie Li, Shouteng Xu, [ ... ], Jiong Hong

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Yi Shen, Yingche Chen, [ ... ], Robert E. Campbell

Directed Chemical Evolution with an Outsized Genetic Code

Casey J. Krusemark, Nicolas P. Tilmans, Patrick O. Brown, Pehr B. Harbury

Directed Evolution and Resolution Mechanism of 1, 3-Propanediol Oxidoreductase from Klebsiella pneumoniae toward Higher Activity by Error-Prone PCR and Bioinformatics

Wei Jiang, Yuan Zhuang, Shizhen Wang, Baishan Fang

Computational Design of the β-Sheet Surface of a Red Fluorescent Protein Allows Control of Protein Oligomerization

Timothy M. Wannier, Matthew M. Moore, Yun Mou, Stephen L. Mayo

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