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Protein engineering

A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, [ ... ], Daisuke Umeno

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold

John D. Steemson, Matthias Baake, [ ... ], Mark T. Liddament

Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, [ ... ], Ziduo Liu

Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

Mechanism of Protein Kinetic Stabilization by Engineered Disulfide Crosslinks

Inmaculada Sanchez-Romero, Antonio Ariza, [ ... ], Jose M. Sanchez-Ruiz

Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

Engineered Monoclonal Antibody with Novel Antigen-Sweeping Activity In Vivo

Tomoyuki Igawa, Atsuhiko Maeda, [ ... ], Kunihiro Hattori

Engineering Agatoxin, a Cystine-Knot Peptide from Spider Venom, as a Molecular Probe for In Vivo Tumor Imaging

Sarah J. Moore, Cheuk Lun Leung, Heidi K. Norton, Jennifer R. Cochran

Identification and Multidimensional Optimization of an Asymmetric Bispecific IgG Antibody Mimicking the Function of Factor VIII Cofactor Activity

Zenjiro Sampei, Tomoyuki Igawa, [ ... ], Kunihiro Hattori

Rational Engineering of a Human Anti-Dengue Antibody through Experimentally Validated Computational Docking

Luca Simonelli, Mattia Pedotti, [ ... ], Luca Varani

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