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Luciferase assay

Deciphering the pathogenicity of three NKX2-1 variants in ultra-severe forms of childhood interstitial lung disease

Yohan David Soreze, Tifenn Desroziers-Louedec, Aurore Carré, Farah Diab, Aphrodite Daskalopoulou, Julie Starck, Valérie Nau, Marie Legendre, Sonia-Athina Karabina, Véronique Houdouin, Aurore Coulomb-L’herminé, Camille Louvrier, Nadia Nathan

published 19 Dec 2025

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1p-Enh-regulated CYP4B1 alleviates NNK-induced heart failure and lung cancer via the STAT3 pathway

Li Dai, Jun Hu, Ye Yang, Bingjun Qian, Xianglin Zuo

published 09 Sep 2025

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LncRNA UCA1 regulates immune micro-environment in cisplatin-induced AKI by miRNA-4498/AKT3 pathway

Peng Hongjun, Lydia Mukanhaire, Liu Zhen, Wang Ting, Li Hongye, Zhang Xiaotian, Liu Guangling, Ren Xianguo

published 12 Feb 2025

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Novel and effective plasmid transfection protocols for functional analysis of genetic elements in human cardiac fibroblasts

Makoto Matsuyama, Takahiro Iwamiya

published 26 Nov 2024

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The endometrial cancer A230V-ALK5 (TGFBR1) mutant attenuates TGF-β signaling and exhibits reduced in vitro sensitivity to ALK5 inhibitors

Eun-Jeong Yu, Daphne W. Bell

published 22 Nov 2024

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A suboptimal OCT4-SOX2 binding site facilitates the naïve-state specific function of a Klf4 enhancer

Jack B. Waite, RuthMabel Boytz, Alexis R. Traeger, Torrey M. Lind, Koya Lumbao-Conradson, Sharon E. Torigoe

published 30 Sep 2024

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Valproic acid use is associated with diminished risk of contracting COVID-19, and diminished disease severity: Epidemiologic and in vitro analysis reveal mechanistic insights

Amanda Watson, Pankil Shah, Doug Lee, Sitai Liang, Geeta Joshi, Ediri Metitiri, Wasim H. Chowdhury, Dean Bacich, Peter Dube, Yan Xiang, Daniel Hanley, Luis Martinez-Sobrido, Ronald Rodriguez

published 02 Aug 2024

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Design and evaluation of antisense sequence length for modified mouse U7 small nuclear RNA to induce efficient pre-messenger RNA splicing modulation in vitro

Takenori Shimo, Otoya Ueda, Satoshi Yamamoto

published 09 Jul 2024

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Circ_0000006 and circ_0000160 regulate hsa-let-7e-5p/UBQLN4 axis in aortic dissection progression

Yong Liu, Liang Wang, Dongyun Lei, Xiong Tan, Weitao Jin, Ming Hou, Kai Hu, Yu Yan, Hao Wang, Chaohu Xiang, Yinglong Lai

published 31 May 2024

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Side-by-side comparison of published small molecule inhibitors against thapsigargin-induced store-operated Ca²⁺ entry in HEK293 cells

Katherine Norman, Karen E. Hemmings, Heba Shawer, Hollie L. Appleby, Alan J. Burnett, Nurasyikin Hamzah, Rajendra Gosain, Emily M. Woodhouse, David J. Beech, Richard Foster, Marc A. Bailey

published 23 Jan 2024

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The RNA-binding KH-domain in the unique transcription factor of the malaria parasite is responsible for its transcriptional regulatory activity

Kanako Komaki-Yasuda, Shigeyuki Kano

published 21 Dec 2023

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Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase

Minami Shichinohe, Shun Ohkawa, Yuu Hirose, Toshihiko Eki

published 22 Nov 2023

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LucFlow: A method to measure Luciferase reporter expression in single cells

Sunil Nooti, Madison Naylor, Trevor Long, Brayden Groll, Manu

published 04 Oct 2023

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