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Guide RNA

Functional role of THRAP3 in modulating thyroid hormone–mediated gene networks in C2C12 myotubes

Taku Fukushima, Sachi Kuse, [ ... ], Iori Sakakibara

Toward an all-in-one recombinant adeno-associated virus vector for functionally ablating the prion gene using CRISPR-Cas technology

Claire Verkuyl, Ari Belotserkovsky, [ ... ], Gerold Schmitt-Ulms

Conformation of HLA-E/peptide complex guides interaction with two novel HLA-E receptors: Stabilin 1 and 2

Tom A. W. Schoufour, Linda Voogd, [ ... ], Simone A. Joosten

Targeting the Exon2 splice cis-element in PD-1 and its effects on lymphocyte function

Yuto Tan, Naoko Kumagai-Takei, [ ... ], Tatsuo Ito

CRISPR-Cas9 targeting the bla_KPC gene in a clinical isolate of Klebsiella michiganensis: Reduction of imipenem resistance and changes in genomic carbapenem resistance determinants

Thaysa Leite Tagliaferri, Alex Krüttgen, [ ... ], Hans-Peter Horz

Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines

Spencer C. Wei, Aaron J. Cantor, [ ... ], Adam P. Silverman

A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

Systematic identification and characterization of high efficiency Cas9 guide RNAs for therapeutic targeting of ADAR

Benjamin G. Gowen, Kory Melton, [ ... ], Spencer C. Wei

DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

Seoee Lee, Hyunyoung Kim, [ ... ], Hyunju Ro

Evidence for gene essentiality in Leishmania using CRISPR

Wen-Wei Zhang, Greg Matlashewski

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

Generation of isogenic models of Angelman syndrome and Prader-Willi syndrome in CRISPR/Cas9-engineered human embryonic stem cells

Rachel B. Gilmore, Dea Gorka, [ ... ], Stormy J. Chamberlain

Proinsulin degradation and presentation of a proinsulin B-chain autoantigen involves ER-associated protein degradation (ERAD)-enzyme UBE2G2

Tom Cremer, Hanneke Hoelen, [ ... ], Emmanuel J. H. J. Wiertz

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