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Guide RNA

Lung development, repair and cancer: A study on the role of MMP20 gene in adenocarcinoma

Pooi Ling Mok, Arun Neela Kumar Anandasayanam, [ ... ], Badr Alzahrani

CaBagE: A Cas9-based Background Elimination strategy for targeted, long-read DNA sequencing

Amelia D. Wallace, Thomas A. Sasani, [ ... ], Aaron R. Quinlan

Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning

Tobias Gross, Csaba Jeney, [ ... ], Stefan Zimmermann

An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes

Cristina Leoni, Niccolò Bianchi, Lucia Vincenzetti, Silvia Monticelli

Efficient multiplexed genome engineering with a polycistronic tRNA and CRISPR guide-RNA reveals an important role of detonator in reproduction of Drosophila melanogaster

Cristin Chon, Grace Chon, [ ... ], Aimin Liu

Enhancer polymorphisms at the IKZF1 susceptibility locus for acute lymphoblastic leukemia impact B-cell proliferation and differentiation in both Down syndrome and non-Down syndrome genetic backgrounds

Vincent U. Gant, Jacob J. Junco, [ ... ], Karen R. Rabin

CRISPR/Cas9-loaded stealth liposomes effectively cleared established HPV16-driven tumours in syngeneic mice

Luqman Jubair, Alfred K. Lam, Sora Fallaha, Nigel A. J. McMillan

Site-specific targeting of a light activated dCas9-KillerRed fusion protein generates transient, localized regions of oxidative DNA damage

Nealia C. M. House, Ramya Parasuram, Jacob V. Layer, Brendan D. Price

Ares-GT: Design of guide RNAs targeting multiple genes for CRISPR-Cas experiments

Eugenio Gómez Minguet

Sensitive and reliable evaluation of single-cut sgRNAs to restore dystrophin by a GFP-reporter assay

Pin Lyu, Kyung Whan Yoo, [ ... ], Baisong Lu

Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells

Louhanna Pinheiro Rodrigues Teixeira, Francisco Eder de Moura Lopes, [ ... ], Kaio Cesar Simiano Tavares

Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site

Stuart J. Smith, Norma Towers, Kim Demetriou, Timothy J. Mohun

Super-enhancer acquisition drives oncogene expression in triple negative breast cancer

Ryan Raisner, Russell Bainer, [ ... ], Karen E. Gascoigne

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