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Enzymes

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Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

Kai Li, Gang Wang,  [ ... ], William T. Pu

Characterization of RNA from Exosomes and Other Extracellular Vesicles Isolated by a Novel Spin Column-Based Method

Daniel Enderle, Alexandra Spiel,  [ ... ], Mikkel Noerholm

A Simplified Method for the Efficient Refolding and Purification of Recombinant Human GM-CSF

Christy A. Thomson, Melanie Olson, Linda M. Jackson, John W. Schrader

Characterization of Bioactive Recombinant Human Lysozyme Expressed in Milk of Cloned Transgenic Cattle

Bin Yang, Jianwu Wang,  [ ... ], Ning Li

De Novo Designed Proteins from a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth

Michael A. Fisher, Kara L. McKinley,  [ ... ], Michael H. Hecht

Garlic Revisited: Antimicrobial Activity of Allicin-Containing Garlic Extracts against Burkholderia cepacia Complex

Daynea Wallock-Richards, Catherine J. Doherty,  [ ... ], Dominic J. Campopiano

Cell-to-Cell Transformation in Escherichia coli: A Novel Type of Natural Transformation Involving Cell-Derived DNA and a Putative Promoting Pheromone

Rika Etchuuya, Miki Ito,  [ ... ], Sumio Maeda

De Novo Enzyme Design Using Rosetta3

Florian Richter, Andrew Leaver-Fay,  [ ... ], David Baker

Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a

Kavleen Sikand, Jagjit Singh, Jey Sabith Ebron, Girish C. Shukla

sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites

Shengsong Xie, Bin Shen,  [ ... ], Yonglian Zhang

Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase

Changlin Fu, William P. Donovan,  [ ... ], Robert H. Cole

Quercetin as a potential treatment for COVID-19-induced acute kidney injury: Based on network pharmacology and molecular docking study

Yue-Yu Gu, Min Zhang,  [ ... ], Hui-Yao Lan

Neutrophil Extracellular Traps Directly Induce Epithelial and Endothelial Cell Death: A Predominant Role of Histones

Mona Saffarzadeh, Christiane Juenemann,  [ ... ], Klaus T. Preissner

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