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DNA construction

Engineering of episomal plasmid structure to enhance non-viral Poly(beta-amino ester) nanoparticle gene delivery to liver and brain cancer cells

Joanna Yang, Jack Kollings, Ethan Idnani, David R. Wilson, Isabella G. Cozzone, Shanelle Mendes, Mahita Varanasi, Stephany Y. Tzeng, Jordan J. Green

published 23 Jul 2026

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Modeling diffuse midline glioma through triple-electrode in utero electroporation of the developing mouse pons

Madisen S. Mason, Hosbaldo Morales Murillo, Madisyn G. Dudek, Samantha E. Maher, Santos J. Franco

published 17 Jun 2026

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Lipid droplet associated protein HILPDA promotes hypoxia-induced ferroptosis by driving LPCAT3-mediated polyunsaturated phospholipids enrichment

Zhenmei Song, Wenli Li, Jie Zeng, Xuexin Wang, Dezhi Wang, Xingchen Liao

published 08 Jun 2026

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Acinetobacter baumannii utilizes a novel protective factor to combat desiccation-induced oxidative stress

John M. Farrow III, Greg Wells, Everett C. Pesci

published 03 Jun 2026

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BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells

Minji Park, Yuree Lim, Yujung Jo, Dong-Hyeon Jo, Sang-Ki Kim, Hyun-Young Kim, Seung-Hwan Lee, Mijeong Lee, Duck Cho

published 21 May 2026

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Practical guidelines for producing non-replicating canine adenovirus vectors

Denis Omara, Christian Ndekezi, Susan Mugaba, Angella Nakyanzi, Henry Bukenya, Josephine Bwogi, Fortunate Natwijuka, Anne Kapaata, Frank Kato, Drake Byamukama, Mercy Lorna Ayebale, David Patrick Kateete, Jennifer Serwanga, Stephen Cose, Sande James Obondo, Pontiano Kaleebu, Sheila Nina Balinda

published 20 May 2026

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Structural dynamics of the human Orai1 channel revealed by cryo-electron microscopy

Yiming Zhang, Yuan Wang, Jindou Liu, Weiwei Bei, Hongkun Wang, Junli Wang, Lei Chen, Youjun Wang

published 11 May 2026

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The wild, wild west of plasmids: First insights into comparative genomics of Borrelia burgdorferi sensu lato

Sabrina Hepner, Robert E. Rollins, Evangelos Mourkas, Konstantin Kuleshov, Sherwood Casjens, Weigang Qiu, Alexandra Dangel, Dunia Velázquez, Sergey Y. Kovalev, Andreas Wieser, Johannes Hübner, Andreas Sing, Volker Fingerle, Gabriele Margos

published 08 May 2026

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Sequencing complete plasmids on Oxford Nanopore Technologies sequencers using R2C2 and Chopper

Kayla D. Schimke, Christopher Vollmers

published 10 Apr 2026

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Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation

James A. Sawitzke, Nina Costantino, Adriana Castillo Caballero, Ellen Hutchinson, Alessandro Barenghi, Lynn C. Thomason, Donald L. Court

published 03 Apr 2026

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Transcriptional and functional characterization of terpene synthase genes of the aromatic plant Plectranthus hadiensis

Hinano Mizuno, Kenro Tokuhiro, Koichiro Iwai, Yoshikazu Furuta, Akari Nakasone, Hidenori Tanaka, Hiroki Sugimoto

published 23 Mar 2026

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A cluster of acidic residues in the cytoplasmic domain of SARS-CoV-2 Spike is required for virion-incorporation and infectivity

Charlotte A. Stoneham, Rajendra Singh, Amalia De Leon, Petra Tafelmeyer, Francisco Acosta, Angus Fuori, Michael Anderson, Peter W. Ramirez, Hannah S. Schwartzer-Sperber, Satish Pillai, Mary K. Lewinski, John Guatelli

published 12 Mar 2026

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TaqMan qPCR quantification of the Cytb gene for precise determination of camel milk in dairy products

Qin Na, Rendalai Si, Liang Ming, Li Yi, Jing He, Tuyatsetseg Jambal, Rimutu Ji

published 25 Feb 2026

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