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DNA manipulations

Research experimental design for the construction and identification of the pGEX-BCKD-E4A recombinant point-mutant plasmid

Tiannan Zhou, Huixian Wei, Jinjun Wang

Characterization of hepatitis B viral forms from patient plasma using velocity gradient: Evidence for an excess of capsids in fractions enriched in Dane particles

Charlotte Pronier, Jérémy Bomo, [ ... ], Vincent Thibault

Enzymatic incorporation of an isotope-labeled adenine into RNA for the study of conformational dynamics by NMR

Hannes Feyrer, Cenk Onur Gurdap, [ ... ], Katja Petzold

CaBagE: A Cas9-based Background Elimination strategy for targeted, long-read DNA sequencing

Amelia D. Wallace, Thomas A. Sasani, [ ... ], Aaron R. Quinlan

A DNA algorithm for the job shop scheduling problem based on the Adleman-Lipton model

Xiang Tian, Xiyu Liu, [ ... ], Yuzhen Zhao

Repurposing a microfluidic formulation device for automated DNA construction

Garima Goyal, Nick Elsbree, [ ... ], Gregory Linshiz

Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

John M. Pryor, Vladimir Potapov, [ ... ], Gregory J. S. Lohman

An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation

Vaishali Verma, Gopal Joshi, Amita Gupta, Vijay K. Chaudhary

Effects of different separation methods on the physical and functional properties of extracellular vesicles

Hyungtaek Jeon, Su-Kyung Kang, Myung-Shin Lee

Maximizing viral detection with SIV droplet digital PCR (ddPCR) assays

Samuel Long, Brian Berkemeier

Pulse-Field capillary electrophoresis of repeat-primed PCR amplicons for analysis of large repeats in Spinocerebellar Ataxia Type 10

Vera Hashem, Anjana Tiwari, [ ... ], Tetsuo Ashizawa

Jmjd1c is dispensable for healthy adult hematopoiesis and Jak2^V617F-driven myeloproliferative disease initiation in mice

Hans F. Staehle, Johannes Heinemann, [ ... ], Jonas Samuel Jutzi

PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations

Izumi Yamaguchi, Takashi Watanabe, Osamu Ohara, Yoshinori Hasegawa

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