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DNA construction

Mal3 is a multi-copy suppressor of the sensitivity to microtubule-depolymerizing drugs and chromosome mis-segregation in a fission yeast pka1 mutant

Takuma Tanabe, Masayuki Yamaga, Makoto Kawamukai, Yasuhiro Matsuo

Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells

Albert Cupo, Victor M. Cruz Portillo, [ ... ], John P. Moore

Suppression of respiratory growth defect of mitochondrial phosphatidylserine decarboxylase deficient mutant by overproduction of Sfh1, a Sec14 homolog, in yeast

Aya Mizuike, Shingo Kobayashi, [ ... ], Ryouichi Fukuda

Purification and biochemical characterization of FrsA protein from Vibrio vulnificus as an esterase

Xiaoqin Wang, Zhi-Min Li, [ ... ], Zhimin Li

An unconventional RNA-based thermosensor within the 5’ UTR of Staphylococcus aureus cidA

Hebaallaha Hussein, Megan E. Fris, [ ... ], Erin R. Murphy

Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

Konstantin B. Ignatov, Konstantin A. Blagodatskikh, [ ... ], Vladimir M. Kramarov

Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not

Benoît J. Pons, Elisabeth Bezine, [ ... ], Gladys Mirey

Modulating the 3’ end-DNA and the fermentation process for enhanced production and biological activity of porcine interferon-gamma

Haiming Cai, Jinbo Deng, [ ... ], Linghua Zhang

A system for production of defective interfering particles in the absence of infectious influenza A virus

Najat Bdeir, Prerna Arora, [ ... ], Michael Winkler

RcsB regulation of the YfdX-mediated acid stress response in Klebsiella pneumoniae CG43S3

Chia-Jui Liu, Ching-Ting Lin, [ ... ], Hwei-Ling Peng

The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods

Jit Ern Chen, Adrian C. Barbrook, [ ... ], Manuel Aranda

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

Saúl Jijón-Moreno, Beatriz Eugenia Baca, Diana Carolina Castro-Fernández, Alberto Ramírez-Mata

PepN is a non-essential, cell wall-localized protein that contributes to neutrophil elastase-mediated killing of Streptococcus pneumoniae

Charmaine N. Nganje, Scott A. Haynes, [ ... ], Mara G. Shainheit

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