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DNA construction

In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran

published 16 Feb 2023

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TFIID dependency of steady-state mRNA transcription altered epigenetically by simultaneous functional loss of Taf1 and Spt3 is Hsp104-dependent

Ryo Iwami, Naoki Takai, Minenosuke Matsutani, Yuh Shiwa, Haruki Kokubo, Koji Kasahara, Tetsuro Kokubo

published 09 Feb 2023

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Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR

Andrew Prantner, Dianna Maar

published 25 Jan 2023

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Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Alisa Nousiainen, Diana Schenkwein, Seppo Ylä-Herttuala

published 20 Jan 2023

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Ectodomain shedding of EGFR ligands serves as an activation readout for TRP channels

Manae Tatsumi, Takayuki Kishi, Satoru Ishida, Hiroki Kawana, Akiharu Uwamizu, Yuki Ono, Kouki Kawakami, Junken Aoki, Asuka Inoue

published 20 Jan 2023

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A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum

Diana G. Calvopina-Chavez, Robyn E. Howarth, Audrey K. Memmott, Oscar H. Pech Gonzalez, Caleb B. Hafen, Kyson T. Jensen, Alex B. Benedict, Jessica D. Altman, Brittany S. Burnside, Justin S. Childs, Samuel W. Dallon, Alexa C. DeMarco, Kirsten C. Flindt, Sarah A. Grover, Elizabeth Heninger, Christina S. Iverson, Abigail K. Johnson, Jack B. Lopez, McKay A. Meinzer, Brook A. Moulder, Rebecca I. Moulton, Hyrum S. Russell, Tiana M. Scott, Yuka Shiobara, Mason D. Taylor, Kathryn E. Tippets, Kayla M. Vainerere, Isabella C. Von Wallwitz, Madison Wagley, Megumi S. Wiley, Naomi J. Young, Joel S. Griffitts

published 04 Jan 2023

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A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus

Steve Swinnen, Christian Zurek, Marco Krämer, Rebecca M. Heger, Jan-Eike Domeyer, Jan Ziegler, Vitali A. Svetlitchnyi, Albrecht Läufer

published 29 Dec 2022

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Efficient production of protein complexes in mammalian cells using a poxvirus vector

Robert Drillien, Karine Pradeau-Aubreton, Julien Batisse, Joëlle Mezher, Emma Schenckbecher, Justine Marguin, Eric Ennifar, Marc Ruff

published 15 Dec 2022

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Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus

Changhui Guan, Peter J. Larson, Elizabeth Fleming, Alexander P. Tikhonov, Sara Mootien, Trudy H. Grossman, Caroline Golino, Julia Oh

published 15 Dec 2022

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Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation

Dana Kolibachuk, Benjamin J. Ryder, Edward R. Lyons, Ariel Woolsey, Margaret K. Lopes, Keya Thakkar, Mayelin Pacheco Nunez, Michaela Duquenoy, Nevan R. Valente, Ashley Nieves

published 07 Dec 2022

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Cas9-mediated endogenous plasmid loss in Borrelia burgdorferi

Constantin N. Takacs, Yuko Nakajima, James E. Haber, Christine Jacobs-Wagner

published 28 Nov 2022

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A genetic approach to identify amino acids in Gcn1 required for Gcn2 activation

Susanne Gottfried, Siaosi M. B. M. J. Koloamatangi, Clement Daube, Anja H. Schiemann, Evelyn Sattlegger

published 28 Nov 2022

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The GGDEF-EAL protein CdgB from Azospirillum baldaniorum Sp245, is a dual function enzyme with potential polar localization

Víctor I. Viruega-Góngora, Iris S. Acatitla-Jácome, David Zamorano-Sánchez, Sandra R. Reyes-Carmona, María L. Xiqui-Vázquez, Beatriz Eugenia Baca, Alberto Ramírez-Mata

published 23 Nov 2022

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