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DNA construction

Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli

Riko Shirakawa, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito

Self-cleaving peptides for expression of multiple genes in Dictyostelium discoideum

Xinwen Zhu, Chiara Ricci-Tam, Emily R. Hager, Allyson E. Sgro

The DNA binding domain and the C-terminal region of DNA Ligase IV specify its role in V(D)J recombination

Vidyasagar Malashetty, Audrey Au, [ ... ], Patricia Cortes

Research experimental design for the construction and identification of the pGEX-BCKD-E4A recombinant point-mutant plasmid

Tiannan Zhou, Huixian Wei, Jinjun Wang

pXOOY: A dual-function vector for expression of membrane proteins in Saccharomyces cerevisiae and Xenopus laevis oocytes

Victoria Amstrup Vold, Sebastian Glanville, Dan Arne Klaerke, Per Amstrup Pedersen

An optimized electrotransformation protocol for Lactobacillus jensenii

Elsa Fristot, Thomas Bessede, [ ... ], Guillaume Cambray

In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran

TFIID dependency of steady-state mRNA transcription altered epigenetically by simultaneous functional loss of Taf1 and Spt3 is Hsp104-dependent

Ryo Iwami, Naoki Takai, [ ... ], Tetsuro Kokubo

Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR

Andrew Prantner, Dianna Maar

Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Alisa Nousiainen, Diana Schenkwein, Seppo Ylä-Herttuala

Ectodomain shedding of EGFR ligands serves as an activation readout for TRP channels

Manae Tatsumi, Takayuki Kishi, [ ... ], Asuka Inoue

A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum

Diana G. Calvopina-Chavez, Robyn E. Howarth, [ ... ], Joel S. Griffitts

A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus

Steve Swinnen, Christian Zurek, [ ... ], Albrecht Läufer

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