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Directed evolution

Effects of selection stringency on the outcomes of directed evolution

Berk A. Alpay, Michael M. Desai

published 14 Oct 2024

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Heuristic algorithms in evolutionary computation and modular organization of biological macromolecules: Applications to in vitro evolution

Alexander V. Spirov, Ekaterina M. Myasnikova

published 27 Jan 2022

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Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology

Roberto Laos, Steven Benner

published 20 Jan 2022

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5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems

Ce Shi, Michael P. Killoran, Mary P. Hall, Paul Otto, Monika G. Wood, Ethan Strauss, Lance P. Encell, Thomas Machleidt, Keith V. Wood, Thomas A. Kirkland

published 14 Dec 2020

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Improved yellow-green split fluorescent proteins for protein labeling and signal amplification

Shuqin Zhou, Siyu Feng, David Brown, Bo Huang

published 23 Nov 2020

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SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

Derek Cerchione, Katherine Loveluck, Eric L. Tillotson, Fred Harbinski, Jen DaSilva, Chase P. Kelley, Elise Keston-Smith, Cecilia A. Fernandez, Vic E. Myer, Hariharan Jayaram, Barrett E. Steinberg

published 16 Apr 2020

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The scale-free nature of protein sequence space

Patrick C. F. Buchholz, Catharina Zeil, Jürgen Pleiss

published 01 Aug 2018

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Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae

Chenlu Zhang, Ligia Acosta-Sampson, Vivian Yaci Yu, Jamie H. D. Cate

published 08 Sep 2017

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Directed evolution provides insight into conformational substrate sampling by SrtA

Muna Suliman, Vishaka Santosh, Tom C. M. Seegar, Annamarie C. Dalton, Kathryn M. Schultz, Candice S. Klug, William A. Barton

published 31 Aug 2017

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Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis

Wenjie Li, Shouteng Xu, Biao Zhang, Yelin Zhu, Yan Hua, Xin Kong, Lianhong Sun, Jiong Hong

published 22 May 2017

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Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Yi Shen, Yingche Chen, Jiahui Wu, Nathan C. Shaner, Robert E. Campbell

published 27 Feb 2017

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Directed Chemical Evolution with an Outsized Genetic Code

Casey J. Krusemark, Nicolas P. Tilmans, Patrick O. Brown, Pehr B. Harbury

published 10 Aug 2016

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Directed Evolution and Resolution Mechanism of 1, 3-Propanediol Oxidoreductase from Klebsiella pneumoniae toward Higher Activity by Error-Prone PCR and Bioinformatics

Wei Jiang, Yuan Zhuang, Shizhen Wang, Baishan Fang

published 03 Nov 2015

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