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Cloning

CRISPR/Cas9 mediated ENT2 gene knockout altered purine catabolic pathway and induced apoptosis in colorectal cell lines

Safaa M. Naes, Sharaniza Ab-Rahim, Musalmah Mazlan, Saiful Effendi Syafruddin, M. Aiman Mohtar, Asmaa Y. Abuhamad, Amirah Abdul Rahman

published 18 Aug 2025

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To be or not to be a virus: A novel chimeric circular Rep-encoding single stranded DNA virus with interfamilial gene exchange illustrates the considerable evolutionary capacity of ssDNA viruses

Sélim Ben Chéhida, Sylvain Lacroix, Murielle Hoareau, Babbitha Fenelon, Arvind Varsani, Darren P. Martin, Pierre-Yves Teycheney, Pierre Lefeuvre, Jean-Michel Lett

published 18 Aug 2025

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Superoxide dismutase VPA1514 in Vibrio parahaemolyticus protects against environmental stresses

Paula Hsu, Hin-chung Wong, Chung-Tao Tang

published 14 Aug 2025

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The FGFR inhibitor pemigatinib overcomes cancer drug resistance to KRAS G12C inhibitors in mesenchymal lung cancer

Angela Abdollahi, Margaret Favata, Michael Weber, Valerie Roman, Rodrigo Hess, Kayla Hammond, Matthew R. Farren, Mike Schaffer, Aidan Gilmartin, Hui Wang, Jonathan Rios-Doria, Alejandro Amador-Arjona

published 11 Aug 2025

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An unexpected role of CLASP1 in radiation response and S-phase regulation of head and neck cancer cells

Reinout H. de Roest, Marijke Buijze, Myrthe Veth, Klaas de Lint, Govind Pai, Martin A. Rooimans, Rob M.F. Wolthuis, Arjen Brink, Jos B. Poell, Ruud H. Brakenhoff

published 06 Aug 2025

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scFv intrabody targeting wildtype TDP-43 presents protective effects in a cellular model of TDP-43 proteinopathy

Yara Al Ojaimi, Rudolf Hergesheimer, Anna A. Chami, Hugo Alarcan, Johanna Augros, Audrey Dangoumau, Shanez Haouari, Jérôme Bourgeais, Antoine Lefevre, Samira Osman, Patrick Emond, Patrick Vourc’h, Christian R. Andres, Philippe Corcia, Olivier Herault, Pierre Martineau, Débora Lanznaster, Hélène Blasco

published 04 Aug 2025

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Cloning and enhancing lumbrokinase production from local Eisenia fetida by signal peptide engineering for effective thrombosis treatment

Adeeba Tabassum, Nadia Zeeshan, Amber Afroz

published 24 Jul 2025

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Extensive novel diversity and phenotypic associations in the dromedary camel microbiome are revealed through deep metagenomics and machine learning

Fathi A. Mubaraki

published 17 Jul 2025

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pET28g: A Golden Gate-compatible pET vector for protein expression in Escherichia coli, validated by production of functional human ACE2

Inês M. Luís, Mariana Parada, João B. Vicente, Isabel A. Abreu

published 07 Jul 2025

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Effects of different clones and inducing time on agarwood quality in grafted Qi-Nan Aquilaria sinensis (Lour.) spreng

Ming Li, Zinong Yang, Zhou Hong, Daping Xu, ZhiHui Li, Shengjie Wang

published 03 Jul 2025

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Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G

Sarshti Kaushik, Sushila Maan, Kanisht Batra, Swati Sindhu, Vijay Kadian, Aman Kumar

published 02 Jul 2025

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Efficient endogenous protein labelling in Dictyostelium using CRISPR/Cas9 knock-in and split fluorescent proteins

Kensuke Yamashita, Tetsuya Muramoto

published 20 Jun 2025

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The dichotomous impacts of Semaphorin3E deficiency on exacerbating airway hyperresponsiveness, remodelling, and inflammation in type-2 low and type-2 high asthma models

Mojdeh Matloubi, Fatemeh Sedaghat, Lianyu Shan, Sujata Basu, Andrew J. Halayko, Abdelilah S. Gounni

published 13 Jun 2025

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