Interpreting Metabolomic Profiles using Unbiased Pathway Models
Figure 2
Active Module Groups from the NGT-EMRN and NGT-CMRN.
Panels (a) and (b) correspond to NGT-EMRN and NGT-CMRN, respectively. Nodes in the AMGs correspond to metabolites in chemical reactions and edges are drawn between reactant-product pairs or shared substrates of enzymes/transporters. A gradient from gold to blue was used to denote reduced percentage change in metabolite abundance after glucose challenge. For clarity, changes were truncated at ±60%. Unmeasured nodes are shown in grey. Edges corresponding to different types of functional links between metabolites are indicated. Cellular locations for metabolites in (a) are assumed to be extracellular unless denoted by [c] for cytoplasmic. Likewise, cellular locations in (b) are assumed to be cytoplasmic unless denoted by [e] for extracellular. The lac-pyr-cit-akg group of metabolites in (a) is connected to the remainder of the set via metabolites with relative frequencies<0.20 across solutions; the same is true of the bile salts cluster in (b).