A Biophysical Model of the Mitochondrial Respiratory System and Oxidative Phosphorylation
Figure 4
Comparison of Model Simulations to Experimental Data on NADH, MVO2, Cytochrome C Redox, and Matrix pH for Model with Phosphate Control
(A) Results for normalized matrix NADH as a function of buffer inorganic phosphate concentration are shown for the two experimental cases of resting mitochondria ([ADP]e = 0, state 4) and active state mitochondria ([ADP]e = 1.3 mM, state 3).
(B) Results for MVO2 (rate of oxygen consumption) are shown for the same experimental cases as in (A).
(C) Results for cytochrome C reduced fraction are shown for the experimental cases as in (A).
(D) Matrix pH (model-simulated and experimentally measured) is plotted as a function of buffer phosphate for the experimental cases as in (A).
All computed results in this figure correspond to steady-state simulations of the model described under “Mitochondrial Model with Phosphate Control.” Model simulations for [ADP]e = 1.3 mM and [ADP]e = 0 mM are plotted as solid lines and dashed lines, respectively; experimental data are the same as plotted in Figure 2.