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November 2021

Primary human foreskin keratinocytes containing HPV18 episomes were grown in a three-dimensional organotypic raft culture to support full epithelial differentiation and the complete HPV18 life cycle. Raft cultures were fixed and paraffin embedded prior to sectioning. Cellular nuclei are stained in blue (DAPI), HPV18 E1^E4 protein (red) and HPV18 L1 protein (green) were stained by indirect immunofluorescence. Ferguson & Campos-León et al.

Image Credit: Parish J, 2021

Editorial

Increasing best practice data sharing at PLOS Pathogens

Lauren Cadwallader, Kasturi Haldar, Michael H. Malim

Opinion

Nasal therapy—The missing link in optimising strategies to improve prevention and treatment of COVID-19

Norman A. Ratcliffe, Helena C. Castro, Izabel C. Paixão, Victor G. O. Evangelho, Patricia Azambuja, Cicero B. Mello

Pearls

Breakthroughs in microbiology made possible with organoids

Carlos J. Ramírez-Flores, Laura J. Knoll

Research Articles

Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1–tomato PTO immune complex via acetylation

Joanna Jeleńska, Jiyoung Lee, Andrew J. Manning, Donald J. Wolfgeher, Youngjoo Ahn, George Walters-Marrah, Ivan E. Lopez, Lissette Garcia, Sheri A. McClerklin, Richard W. Michelmore, Stephen J. Kron, Jean T. Greenberg

Rv0180c contributes to Mycobacterium tuberculosis cell shape and to infectivity in mice and macrophages

Delphine Payros, Henar Alonso, Wladimir Malaga, Arnaud Volle, Serge Mazères, Sébastien Déjean, Sophie Valière, Flavie Moreau, Stéphanie Balor, Alexandre Stella, Lucie Combes-Soia, Odile Burlet-Schiltz, Olivier Bouchez, Jérôme Nigou, Catherine Astarie-Dequeker, Christophe Guilhot

The chromatin insulator CTCF regulates HPV18 transcript splicing and differentiation-dependent late gene expression

Jack Ferguson, Karen Campos-León, Ieisha Pentland, Joanne D. Stockton, Thomas Günther, Andrew D. Beggs, Adam Grundhoff, Sally Roberts, Boris Noyvert, Joanna L. Parish

Evidence of MHC class I and II influencing viral and helminth infection via the microbiome in a non-human primate

B. Karina Montero, Wasimuddin, Nina Schwensow, Mark A. F. Gillingham, Yedidya R. Ratovonamana, S. Jacques Rakotondranary, Victor Corman, Christian Drosten, Jörg U. Ganzhorn, Simone Sommer

Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis

Paulo Vieira, Roxana Y. Myers, Clement Pellegrin, Catherine Wram, Cedar Hesse, Thomas R. Maier, Jonathan Shao, Georgios D. Koutsovoulos, Inga Zasada, Tracie Matsumoto, Etienne G. J. Danchin, Thomas J. Baum, Sebastian Eves-van den Akker, Lev G. Nemchinov

Species-specific quantification of circulating ebolavirus burden using VP40-derived peptide variants

Qingbo Shu, Tara Kenny, Jia Fan, Christopher J. Lyon, Lisa H. Cazares, Tony Y. Hu

The P. falciparum CSP repeat region contains three distinct epitopes required for protection by antibodies in vivo

Yevel Flores-Garcia, Lawrence T. Wang, Minah Park, Beejan Asady, Azza H. Idris, Neville K. Kisalu, Christian Muñoz, Lais S. Pereira, Joseph R. Francica, Robert A. Seder, Fidel Zavala

ΔNp63α promotes Epstein-Barr virus latency in undifferentiated epithelial cells

Nicholas Van Sciver, Makoto Ohashi, Dhananjay M. Nawandar, Nicholas P. Pauly, Denis Lee, Kathleen R. Makielski, Jillian A. Bristol, Sai Wah Tsao, Paul F. Lambert, Eric C. Johannsen, Shannon C. Kenney

Mutation Y453F in the spike protein of SARS-CoV-2 enhances interaction with the mink ACE2 receptor for host adaption

Wenlin Ren, Jun Lan, Xiaohui Ju, Mingli Gong, Quanxin Long, Zihui Zhu, Yanying Yu, Jianping Wu, Jin Zhong, Rong Zhang, Shilong Fan, Guocai Zhong, Ailong Huang, Xinquan Wang, Qiang Ding

Suppression of inflammatory arthritis by the parasitic worm product ES-62 is associated with epigenetic changes in synovial fibroblasts

Marlene Corbet, Miguel A. Pineda, Kun Yang, Anuradha Tarafdar, Sarah McGrath, Rinako Nakagawa, Felicity E. Lumb, Colin J. Suckling, William Harnett, Margaret M. Harnett

Impact of extrinsic incubation temperature on natural selection during Zika virus infection of Aedes aegypti and Aedes albopictus

Reyes A. Murrieta, Selene M. Garcia-Luna, Deedra J. Murrieta, Gareth Halladay, Michael C. Young, Joseph R. Fauver, Alex Gendernalik, James Weger-Lucarelli, Claudia Rückert, Gregory D. Ebel

Polymerization of C9 enhances bacterial cell envelope damage and killing by membrane attack complex pores

Dennis J. Doorduijn, Dani A. C. Heesterbeek, Maartje Ruyken, Carla J. C. de Haas, Daphne A. C. Stapels, Piet C. Aerts, Suzan H. M. Rooijakkers, Bart W. Bardoel

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Corrections

Correction: RNF111-facilitated neddylation potentiates cGAS-mediated antiviral innate immune response

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