Wang Qiang and Colleagues have used Sonchus yellow net rhabdovirus (SYNV) to develop the first reverse genetics system reported for a plant negative strand RNA virus. The SYNV genome was cloned and a derivative expressing GFP (rSYNV-GFP) was constructed and used to infect plants. The image shows GFP fluorescence against a red chloroplast autofluorescence background in a Nicotiana benthamiana plant systemically infected with rSYNV-GFP. Li et al.
Image Credit: Li et al.
Opinions
Effectively Communicating the Uncertainties Surrounding Ebola Virus Transmission
PLOS Pathogens: published October 29, 2015 | https://doi.org/10.1371/journal.ppat.1005097
Addressing the Complications of Ebola and Other Viral Hemorrhagic Fever Infections: Using Insights from Bacterial and Fungal Sepsis
PLOS Pathogens: published October 1, 2015 | https://doi.org/10.1371/journal.ppat.1005088
Pearls
Dimensions of Horizontal Gene Transfer in Eukaryotic Microbial Pathogens
PLOS Pathogens: published October 29, 2015 | https://doi.org/10.1371/journal.ppat.1005156
Time for Chocolate: Current Understanding and New Perspectives on Cacao Witches’ Broom Disease Research
PLOS Pathogens: published October 22, 2015 | https://doi.org/10.1371/journal.ppat.1005130
Papillomavirus E6 Oncoproteins Take Common Structural Approaches to Solve Different Biological Problems
PLOS Pathogens: published October 15, 2015 | https://doi.org/10.1371/journal.ppat.1005138
A Gut Odyssey: The Impact of the Microbiota on Clostridium difficile Spore Formation and Germination
PLOS Pathogens: published October 15, 2015 | https://doi.org/10.1371/journal.ppat.1005157
Chronobiomics: The Biological Clock as a New Principle in Host–Microbial Interactions
PLOS Pathogens: published October 8, 2015 | https://doi.org/10.1371/journal.ppat.1005113
Interferon-γ: The Jekyll and Hyde of Malaria
PLOS Pathogens: published October 1, 2015 | https://doi.org/10.1371/journal.ppat.1005118
Research Matters
Preparing for the Next Epidemic with Basic Virology
PLOS Pathogens: published October 22, 2015 | https://doi.org/10.1371/journal.ppat.1005182
Translating Basic Research into Clinical Applications: Malaria Research at an NIH Lab
PLOS Pathogens: published October 8, 2015 | https://doi.org/10.1371/journal.ppat.1005190
Research Articles
A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo
PLOS Pathogens: published October 30, 2015 | https://doi.org/10.1371/journal.ppat.1005238
Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable Vaccine
PLOS Pathogens: published October 29, 2015 | https://doi.org/10.1371/journal.ppat.1005215
Calcium Regulation of Hemorrhagic Fever Virus Budding: Mechanistic Implications for Host-Oriented Therapeutic Intervention
PLOS Pathogens: published October 29, 2015 | https://doi.org/10.1371/journal.ppat.1005220
C-di-GMP Regulates Motile to Sessile Transition by Modulating MshA Pili Biogenesis and Near-Surface Motility Behavior in Vibrio cholerae
PLOS Pathogens: published October 27, 2015 | https://doi.org/10.1371/journal.ppat.1005068
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Structure Analysis Uncovers a Highly Diverse but Structurally Conserved Effector Family in Phytopathogenic Fungi
PLOS Pathogens: published October 27, 2015 | https://doi.org/10.1371/journal.ppat.1005228
Systematic Identification of Cyclic-di-GMP Binding Proteins in Vibrio cholerae Reveals a Novel Class of Cyclic-di-GMP-Binding ATPases Associated with Type II Secretion Systems
PLOS Pathogens: published October 27, 2015 | https://doi.org/10.1371/journal.ppat.1005232
Related Articles
Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins
PLOS Pathogens: published October 27, 2015 | https://doi.org/10.1371/journal.ppat.1005244
Related Articles
TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus
PLOS Pathogens: published October 27, 2015 | https://doi.org/10.1371/journal.ppat.1005253
Modeling the Effects of Vorinostat In Vivo Reveals both Transient and Delayed HIV Transcriptional Activation and Minimal Killing of Latently Infected Cells
PLOS Pathogens: published October 23, 2015 | https://doi.org/10.1371/journal.ppat.1005237
Identification of a Novel Lipoprotein Regulator of Clostridium difficile Spore Germination
PLOS Pathogens: published October 23, 2015 | https://doi.org/10.1371/journal.ppat.1005239
Yersinia pestis Requires Host Rab1b for Survival in Macrophages
PLOS Pathogens: published October 23, 2015 | https://doi.org/10.1371/journal.ppat.1005241
Immunologic Control of Mus musculus Papillomavirus Type 1
PLOS Pathogens: published October 23, 2015 | https://doi.org/10.1371/journal.ppat.1005243
The Fungal Exopolysaccharide Galactosaminogalactan Mediates Virulence by Enhancing Resistance to Neutrophil Extracellular Traps
PLOS Pathogens: published October 22, 2015 | https://doi.org/10.1371/journal.ppat.1005187
Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein
PLOS Pathogens: published October 22, 2015 | https://doi.org/10.1371/journal.ppat.1005202
Crystal Structures of a Piscine Betanodavirus: Mechanisms of Capsid Assembly and Viral Infection
PLOS Pathogens: published October 22, 2015 | https://doi.org/10.1371/journal.ppat.1005203
Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes
PLOS Pathogens: published October 22, 2015 | https://doi.org/10.1371/journal.ppat.1005236
Related Articles
Depletion of M. tuberculosis GlmU from Infected Murine Lungs Effects the Clearance of the Pathogen
PLOS Pathogens: published October 21, 2015 | https://doi.org/10.1371/journal.ppat.1005235
Structure Elucidation of Coxsackievirus A16 in Complex with GPP3 Informs a Systematic Review of Highly Potent Capsid Binders to Enteroviruses
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005165
CD39 Expression Identifies Terminally Exhausted CD8+ T Cells
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005177
Carcinogenic Parasite Secretes Growth Factor That Accelerates Wound Healing and Potentially Promotes Neoplasia
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005209
Phenotypic and Functional Alterations in Circulating Memory CD8 T Cells with Time after Primary Infection
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005219
Dissociation of Tissue Destruction and Bacterial Expansion during Bubonic Plague
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005222
Rescue of a Plant Negative-Strand RNA Virus from Cloned cDNA: Insights into Enveloped Plant Virus Movement and Morphogenesis
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005223
Related Articles
PD-L1 Expression on Retrovirus-Infected Cells Mediates Immune Escape from CD8+ T Cell Killing
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005224
Related Articles
Crystal Structure of the Human Cytomegalovirus Glycoprotein B
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005227
Related Articles
Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005230
Abiotic Stresses Antagonize the Rice Defence Pathway through the Tyrosine-Dephosphorylation of OsMPK6
PLOS Pathogens: published October 20, 2015 | https://doi.org/10.1371/journal.ppat.1005231
Structural and Functional Analysis of Murine Polyomavirus Capsid Proteins Establish the Determinants of Ligand Recognition and Pathogenicity
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005104
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Ganglioside and Non-ganglioside Mediated Host Responses to the Mouse Polyomavirus
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005175
Related Articles
Shigella Effector OspB Activates mTORC1 in a Manner That Depends on IQGAP1 and Promotes Cell Proliferation
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005200
Peripheral Vγ9Vδ2 T Cells Are a Novel Reservoir of Latent HIV Infection
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005201
Fundamental Roles of the Golgi-Associated Toxoplasma Aspartyl Protease, ASP5, at the Host-Parasite Interface
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005211
Respiratory Syncytial Virus Disease Is Mediated by Age-Variable IL-33
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005217
The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways
PLOS Pathogens: published October 16, 2015 | https://doi.org/10.1371/journal.ppat.1005218
Distinct Viral and Mutational Spectrum of Endemic Burkitt Lymphoma
PLOS Pathogens: published October 15, 2015 | https://doi.org/10.1371/journal.ppat.1005158
CCR2+ Inflammatory Dendritic Cells and Translocation of Antigen by Type III Secretion Are Required for the Exceptionally Large CD8+ T Cell Response to the Protective YopE69-77 Epitope during Yersinia Infection
PLOS Pathogens: published October 15, 2015 | https://doi.org/10.1371/journal.ppat.1005167
The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy
PLOS Pathogens: published October 9, 2015 | https://doi.org/10.1371/journal.ppat.1005174
Related Articles
Comparative Life Cycle Transcriptomics Revises Leishmania mexicana Genome Annotation and Links a Chromosome Duplication with Parasitism of Vertebrates
PLOS Pathogens: published October 9, 2015 | https://doi.org/10.1371/journal.ppat.1005186
IL-4 Induced Innate CD8+ T Cells Control Persistent Viral Infection
PLOS Pathogens: published October 9, 2015 | https://doi.org/10.1371/journal.ppat.1005193
Influenza Transmission in the Mother-Infant Dyad Leads to Severe Disease, Mammary Gland Infection, and Pathogenesis by Regulating Host Responses
PLOS Pathogens: published October 8, 2015 | https://doi.org/10.1371/journal.ppat.1005173
Influenza Virus Reassortment Is Enhanced by Semi-infectious Particles but Can Be Suppressed by Defective Interfering Particles
PLOS Pathogens: published October 6, 2015 | https://doi.org/10.1371/journal.ppat.1005204
BCG Skin Infection Triggers IL-1R-MyD88-Dependent Migration of EpCAMlow CD11bhigh Skin Dendritic cells to Draining Lymph Node During CD4+ T-Cell Priming
PLOS Pathogens: published October 6, 2015 | https://doi.org/10.1371/journal.ppat.1005206
Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells
PLOS Pathogens: published October 5, 2015 | https://doi.org/10.1371/journal.ppat.1005191
The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005123
Antigen-Specific Th17 Cells Are Primed by Distinct and Complementary Dendritic Cell Subsets in Oropharyngeal Candidiasis
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005164
Trans-generational Immune Priming Protects the Eggs Only against Gram-Positive Bacteria in the Mealworm Beetle
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005178
The Suramin Derivative NF449 Interacts with the 5-fold Vertex of the Enterovirus A71 Capsid to Prevent Virus Attachment to PSGL-1 and Heparan Sulfate
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005184
Disruption of Sphingolipid Biosynthesis Blocks Phagocytosis of Candida albicans
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005188
Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005194
Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005195
Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005196
The Timing of Stimulation and IL-2 Signaling Regulate Secondary CD8 T Cell Responses
PLOS Pathogens: published October 2, 2015 | https://doi.org/10.1371/journal.ppat.1005199
The White-Nose Syndrome Transcriptome: Activation of Anti-fungal Host Responses in Wing Tissue of Hibernating Little Brown Myotis
PLOS Pathogens: published October 1, 2015 | https://doi.org/10.1371/journal.ppat.1005168
Expression of Concern
Expression of Concern: Misregulation of AUXIN RESPONSE FACTOR 8 Underlies the Developmental Abnormalities Caused by Three Distinct Viral Silencing Suppressors in Arabidopsis
PLOS Pathogens: published October 9, 2015 | https://doi.org/10.1371/journal.ppat.1005234