S10 Fig. The representative images of vIRF1-induced cell motility, plate colony formation and in vivo angiogenesis with knockdown of GLRX3.

(A). GLRX3 was interfered by two different shRNAs in vIRF1 transduced pri-HUVECs. Cells were subjected to Transwell migration and invasion assay described in the “Materials and methods” section. The migrated and invaded cells were counted at 6 h and 12 h post seeding. Representational photographs of migration and invasion were exhibited (original magnification, × 100). Quantification of Transwell migration and invasion assay was described in Fig 7H and 7I. (B). Plate colony formation assay of EA.hy926 cells treated as in (A) was performed as described in the “Materials and methods” section. Representational photographs of plate colony were exhibited. Quantification of plate colony formation assay was described in Fig 7J. (C). The mixture containing high concentration Matrigel and EA.hy926 cells treated as in (A) was injected into nude mice. The details were shown in the “Materials and methods” section. Representational photographs of plugs were exhibited. Scar bars, 1 cm. Quantification of hemoglobin in plug tissues was described in Fig 7K.

https://doi.org/10.1371/journal.ppat.1014267.s001

(EPS)