https://orcid.org/0000-0003-4740-6991
Figures
Abstract
Several reports suggest that intestinal tissue may be a natural niche for Chlamydia trachomatis infection and a reservoir for persistent infections in the human body. Due to the human specificity of the pathogen and the lack of suitable host models, there is limited knowledge on this topic. In our study, we modelled the course of the chlamydial infection in human primary gastrointestinal (GI) epithelial cells originating from patient-derived organoids. We show that GI cells are resistant to apical infection and C. trachomatis needs access to the basolateral membrane to establish an infection. Transmission electron microscopy analysis reveals the presence of both normal as well as aberrant chlamydial developmental forms in the infected cells, suggesting a possible cell-type specific nature of the infection. Furthermore, we show that the plasmid-encoded Pgp3 is an important virulence factor for the infection of human GI cells. This is the first report of C. trachomatis infection in human primary intestinal epithelial cells supporting a possible niche for chlamydial infection in the human intestinal tissue.
Author summary
Chlamydial infection has a high global prevalence and is a major health concern. Untreated infections may cause complications and lead to serious health problems, especially in women. Although the infection is usually localized to the genital tract, experiments performed in a mouse infection model as well as the accumulating clinical data suggest that the human gastrointestinal (GI) tract might represent a hidden infection niche and a source of reinfections. In our study, we used the advantages of the organoid technology to model the chlamydial infection in patient-derived primary GI epithelial cells. We were able to show that these cells are resistant to the infection, however, Chlamydia could utilize a basolateral entry route for efficient infection. Chlamydia form either normal or persistent-like developmental forms in these GI epithelial cells. We also showed the importance of the plasmid-mediated virulence in the infection of human GI cells. The results obtained in the GI infection model replicated phenotypes predicted and expected for Chlamydia human intestinal infection, and therefore support a role of the human GI tract as a potential niche for chlamydial infection.
Citation: Hovhannisyan P, Stelzner K, Keicher M, Paprotka K, Neyazi M, Pauzuolis M, et al. (2024) Infection of human organoids supports an intestinal niche for Chlamydia trachomatis. PLoS Pathog 20(8): e1012144. https://doi.org/10.1371/journal.ppat.1012144
Editor: Guangming Zhong, University of Texas Health Science Center at San Antonio, UNITED STATES OF AMERICA
Received: March 21, 2024; Accepted: July 15, 2024; Published: August 22, 2024
Copyright: © 2024 Hovhannisyan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the manuscript and its Supporting Information files.
Funding: This work was funded by the Deutsche Forschungsgemeinschaft (DFG) in the RTG 2157/2 3D Infect and the SFB 1583 DECIDE to S.B., T.R. and the European Research Council (grant no. ERC-2018-ADG/NCI-CAD) to T.R. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Chlamydia trachomatis is a human-specific pathogen, which causes the most common bacterial sexually transmitted infections worldwide [1]. Different serovars of C. trachomatis have specific tissue tropism and cause diseases at different anatomical sites: serovars A-C cause eye infections, genital infections are usually associated with the serovars D-K and the more invasive serovars L1-L3 infect the lymphatic system [2,3].
Chlamydia are obligate intracellular bacteria with a unique biphasic developmental cycle, during which they alternate between two morphologically and functionally different forms–elementary bodies (EBs) and reticulate bodies (RBs). Chlamydia possess complex and redundant mechanisms for host cell attachment and entry, which explains their ability to infect a wide range of cell types [4]. Several host cell receptors, including human integrin β1 receptor [5], epidermal growth factor receptor [6], fibroblast growth factor receptor [7] and Ephrin A2 receptor (EphA2) [8], have been found to be used by C. trachomatis to enter the host cell. EBs, the infectious forms of the organism, are adapted to survive in extracellular space [9]. Upon contact with the host cell, they induce their internalization and develop in a membrane bound compartment called inclusion, where they differentiate into RBs. After several rounds of replication, RBs re-differentiate into EBs, which are released from the cell to infect neighboring cells [9,10]. Under stress conditions, RBs can enter a non-replicative but viable state called persistence, in order to survive the unfavorable conditions. They can re-enter the developmental cycle after the physiological conditions have normalized [1,10].
The obligate intracellular lifestyle and human specificity of C. trachomatis limit the availability of relevant physiological host models to study the infection. Most of the knowledge about the interactions of C. trachomatis with the host is derived from in vitro experiments, which are mainly based on the use of transformed cell line models and which not always recapitulate the in vivo situation [11]. Since it is possible to infect mice with human C. trachomatis serovars under certain conditions, murine models have been widely used to study the immunopathogenesis. However, these do not always reflect the pathology of the disease observed in humans [12,13]. In recent years, the advances in stem cell biology allowed the establishment of complex human primary cell-based host models, such as organoids, which are currently being actively used in the field of infection biology [14,15]. Chlamydial infection has been recently successfully modelled and studied in both human and murine organoids derived from female genital tract tissues, such as human fallopian tube organoids [16], murine endometrial organoids [17] and human cervical organoids [18]. These studies have profoundly improved our understanding of chlamydial infection.
The majority of studies on C. trachomatis-host interactions focuses on the genital tract. There is only a limited number of studies addressing the infection at extra-genital sites. It is well known that C. trachomatis can infect the epithelium of the human rectum and pharynx, with a high prevalence in men who have sex with men [19]. There have also been reported cases of C. trachomatis DNA and antigens being detected in appendix and intestinal biopsies from patients [20,21].
Besides human-specific pathogens, the genus Chlamydia contains species, which infect wild or domesticated animals [22]. Interestingly, gastrointestinal (GI) infection occurs in most animal hosts and the GI tract is a natural site of Chlamydia infection [23]. Some authors have proposed that C. trachomatis could have evolved as a commensal of the human GI tract [24] and that the human GI tract can be a site of persistent chlamydial infections and a possible reservoir of infections in the genital tract [23,25]. Studies in mice demonstrated that following oral inoculation, the murine chlamydial pathogen C. muridarum crosses multiple GI barriers and establishes a long lasting non-pathological colonization in the large intestine [26]. It was also reported that Pgp3, a chlamydial plasmid-encoded virulence factor, is important for the colonization of the GI tract of mice as it helps Chlamydia to reach the large intestine by providing resistance against gastric acid in stomach and CD4+ T lymphocyte-mediated immunity in the small intestine [26].
In the present work, we investigated C. trachomatis infection in primary epithelial cells derived from different regions of the human GI tract using organoid-based host models. We show that C. trachomatis is able to infect the human GI cells from the basolateral, but not apical surface. Moreover, we demonstrate that in some cells chlamydial development is restricted and leads to the formation of aberrant bodies, hallmarks of persistent infection. We also reveal that the chlamydial plasmid and plasmid-encoded Pgp3 are important virulence factors for the infection of human GI cells as their absence leads to growth defects.
Results
C. trachomatis infects human primary GI epithelial cells
To test if C. trachomatis has the ability to infect human primary GI epithelial cells, we used human adult stem cell-derived organoids as a host model. They consist of primary epithelial cells organized into a single layer. In order to have each major region of the human GI tract represented in our studies, we modelled the infection in organoids derived from human corpus (stomach), jejunum (small intestine) and colon (large intestine) (Figs 1A and S1). GI organoids were dissociated into small multicellular fragments, infected with GFP-expressing C. trachomatis and re-suspended into fresh extracellular matrix (ECM) for the formation of new organoids. Starting from 24 hours post-infection (p.i.), we could clearly observe fluorescent C. trachomatis inclusions in both gastric and intestinal organoids (Fig 1B). However, in order to avoid the shredding of the 3D organoids, which disrupts the epithelial barrier integrity and to mimic the natural (apical) route of infection, we switched to a 2D setting by generating monolayers from organoids. In 2D configuration, GI cells grow in a patchy manner by forming dense small islands (S1 Fig), which gradually grow and eventually fuse forming a confluent monolayer. We infected the GI cells and HeLa cells (a common C. trachomatis host model) with C. trachomatis and observed that, in contrast to HeLa cells, chlamydial inclusions in the GI monolayers exhibited uneven distribution and were mostly located on the edges of the cell islands (Fig 1C). The infection pattern was maintained even after several rounds of chlamydial replication (4 and 8 days p.i.) and the inclusions were mostly observed on the edges of cell patches or at the fusion of two or more cell patches. We also observed that the infection progresses from the margins inward to the centre of the patches, presumably via successive infection of new adjacent cells. While HeLa cells were almost completely lysed by C. trachomatis infection on day 4, primary GI cells, especially those in the centre of the monolayers, were still intact.
(A) Schematic representation of host model generation. (B) Human gastric and intestinal organoids infected with GFP-expressing C. trachomatis (MOI of 5) at 48 hours p.i. (C) Organoid-derived subconfluent monolayers of human primary gastrointestinal epithelial cells and HeLa cells were infected with GFP-expressing C. trachomatis (MOI of 0.5) and observed daily. Shown are representative images obtained at 1, 4 and 8 days p.i.. Images in (B) and (C) were taken with phase-contrast and in the green fluorescence channel and merged. Scale bar: 100 μm. Fig 1A was prepared in Biorender.com.
Disruption of the cell junctions increases the efficiency of C. trachomatis infection in GI cells
The observed novel pattern of C. trachomatis infection in GI monolayers could either be attributable to the physical properties of cells in monolayers or be a consequence of cell type/state-specific infection events. As we observed that proliferative cells are often found closer to the edges of cell islands in GI monolayers, we asked whether Chlamydia might predominantly infect actively dividing cells. We co-stained the infected gastric monolayers for Ki67, which is a nuclear protein and a marker of proliferation, and determined the number of inclusions associated with Ki67-positive or negative cells (S2A and S2B Fig). We could not find a direct link between the cell proliferation status and the infection, as the microscopic analysis revealed a similar infection burden in Ki67-positive and negative cells.
We also observed that, in contrast to HeLa cells, GI monolayer cells have a dense arrangement within individual cell patches and hypothesized that Chlamydia might preferentially infect the edges of the patches due to the distinct localization of the entry receptors on the basolateral surface, e.g. at cell-cell junctions, and their absence on the apical surface of the cells. To test this hypothesis, we disrupted the cell-cell junctions by pre-treating the gastric cells with the Ca2+ chelating agent ethylene glycol-bis(beta-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA), which was previously reported to cause disruption of tight junctions and increase the paracellular space [27,28]. We applied live cell imaging to monitor the effect of EGTA on primary gastric and HeLa cells. Characteristic morphological changes, such as gradually increasing distance between neighbouring cells and loss of monolayer integrity, were observed in the treated gastric cells, whereas HeLa cells exhibited a more defined and elongated shape (Fig 2A). Following EGTA pre-treatment, gastric and HeLa cells were infected with C. trachomatis and subjected to microscopy analysis 24 hours p.i. It revealed a more dispersed infection pattern in the EGTA-treated gastric cells compared to untreated control cells (Fig 2B), as well as a significantly higher infection rate in gastric cells pre-treated with EGTA for 30 min (approx. 1.7-fold higher) or 60 min (approx. 2.8-fold higher) (Fig 2C). We detected no changes in the infection pattern or rate of HeLa cells upon pre-treatment with EGTA (Fig 2B and 2C). The cell morphology and the infection pattern of intestinal cells were affected in a similar manner upon EGTA pre-treatment (S3A and S3B Fig).
(A) Stills from live cell imaging experiment performed to monitor the effect of 4 mM EGTA on the morphology of human gastric (left panel) and HeLa (right panel) cells over the time course of treatment (green: CellTracker dye, gray: brightfield). Scale bar: 25 μm. (B) Representative confocal fluorescence images showing the effect of EGTA treatment on the outcome of infection in gastric (left panel) and HeLa (right panel) cells. Gastric and HeLa cells were pre-treated with 4 mM EGTA (for 15, 30 or 60 min) or left untreated, and infected with GFP-expressing C. trachomatis (MOI of 5 and 0.5, respectively). 24 hours p.i. the cells were fixed, stained and subjected to confocal microscopy (blue: DAPI, green: C. trachomatis, red: E-Cadherin or Actin). Scale bar: 50 μm. (C) To measure the changes of the infection rate caused by EGTA pre-treatment, the number of chlamydial inclusions and host cell nuclei was determined in 14 fields of view per sample by automated microscopy. Data represent the mean ± SD from three independent experiments. Statistical analysis was performed by two-way ANOVA (ns = not significant, *P<0.05, ****P<0.0001).
C. trachomatis infects the GI cells via the basolateral route
As GI cells with disrupted junctions could be efficiently infected by C. trachomatis, we next tested the ability of the pathogen to selectively infect gastric and intestinal monolayers from the apical versus the basolateral surface. Culturing the cells on a porous membrane in cell culture inserts allowed a separation of these two surfaces. Gastric and small intestinal primary cells were seeded on the membrane and grown until they formed a confluent monolayer with a polarized phenotype, where the basolateral side of the cells was oriented to the cell culture insert membrane. The cells were infected with C. trachomatis from the apical or basolateral side (Fig 3A) and analyzed 24 hours p.i. by confocal microscopy. Interestingly, we detected no or extremely few inclusions in apically infected gastric and intestinal monolayers, whereas the basolateral infection was highly efficient (Figs 3B and S4A). To check whether the observed phenotype of basolateral infection is specific to human GI cells only or might also be common for other columnar epithelial cell types, we performed the infection assay in organoid-derived human primary fallopian tube epithelial cells (S4B and S4C Fig). Although we detected inclusions in apically infected fallopian tube samples, the basolateral infection resulted in a significantly higher infection rate.
(A) Schematic representation of the infection setup. (B) Gastric and small intestinal cells grown on cell culture inserts were infected with GFP-expressing C. trachomatis (MOI of 5) from the apical or basolateral surface and after 2 hours of incubation the inoculum was removed, and the cells were kept in fresh organoid medium. 24 hours p.i. the cells were fixed, stained and subjected to confocal microscopy. Shown are representative confocal fluorescence Z-stack images of at least three independent experiments (blue: DAPI, green: C. trachomatis). Scale bar: 50 μm. (C) Representative confocal microscopy image showing the basolateral localization of the integrin β1 receptor in the cross-section of polarized human primary gastric cells. Human primary gastric cells were cultured on a porous polycarbonate membrane in a cell culture insert to generate a confluent and polarized monolayer. 5 μm histological cross-sections of the PFA-fixed and paraffin-embedded samples were used for the immunofluorescence analysis (blue: DAPI, green: integrin β1, red: E-cadherin). The fluorescence channels were merged with the bright field channel to visualize cell orientation. Scale bar: 10 μm. (D) The gastric cells grown on cell culture inserts were pre-treated with 25 μg/ml blocking anti-integrin β1 antibodies for 1 hour or left untreated, infected with GFP-expressing C. trachomatis (MOI of 5) for 2 hours in the presence of blocking antibodies. 24 hours p.i. cells were fixed, stained and subjected to confocal microscopy. Shown are representative confocal fluorescence Z-stack images of five independent experiments (blue: DAPI, green: C. trachomatis). Scale bar: 50 μm. (E) The number of chlamydial inclusions and host cell nuclei in (D) was quantified in at least 10 fields of view per sample using Fiji. Data represent the mean ± SD from five independent experiments. Statistical analysis was performed by unpaired t-test (***P<0.001). Fig 3A was prepared in Biorender.com.
Some authors have suggested that the mostly submucosal, invasive LGV strains and the non-invasive genital strains of C. trachomatis may differ in their tropism for cellular domains and thus preferentially target the basolateral and apical surfaces of the columnar epithelial cells, accordingly [29]. To address this possibility and to test whether the observed basolateral entry route is also used by the genital serovars, we infected the polarized gastric cells with C. trachomatis serovar E and serovar K and 24 hours p.i. compared the outcomes of apical versus basolateral infections. Microscopic analysis revealed an infection pattern similar to that of LGV serovar L2 and a significantly higher infection rate during the basolateral infection for both genital serovars (S4D and S4E Fig).
Next, we aimed to identify a basolaterally expressed host cell receptor, which could potentially be involved in the invasion of GI cells by C. trachomatis. Integrin receptors, including β1-integrins, are usually located at the basolateral surface of polarized epithelial cells [30,31]. Integrin β1 receptor is also exploited by C. trachomatis for host cell entry among several other receptors [5]. Immunohistological analysis confirmed the distinct basolateral localization of the integrin β1 receptor and its absence on the apical surface of the polarized gastric cells (Fig 3C). To determine its involvement in the infection, we treated the gastric cells grown on cell culture inserts with anti-integrin β1 blocking antibodies and infected with C. trachomatis. Confocal microscopy analysis of the infected samples 24 hours p.i. revealed a significantly reduced (2.3-fold) infection rate in antibody-treated cells compared to untreated cells (Fig 3D and 3E). Collectively, our findings indicate that human GI epithelial cells are highly resistant to C. trachomatis apical infection and an access to the receptors localized on the basolateral surface of the cells is needed for efficient infection.
Chlamydial plasmid-encoded Pgp3 is important for propagation in human GI cells
The chlamydial plasmid, particularly the plasmid-encoded virulence factor Pgp3, is important for the colonization of the GI tract of the mice by C. muridarum [32]. Pgp3 has been found to play a critical role in protecting Chlamydia against gastric acid killing and thus allowing its further dissemination into the lower GI tract of the mice. We asked whether chlamydial plasmid and Pgp3 are involved in the infection of human GI cells and therefore compared the infectivity of wild-type (Ctr WT), Pgp3-deficient (Ctr Δpgp3) and plasmid-free (Ctr PF) C. trachomatis strains in human gastric, small and large intestinal epithelial cells (Fig 4A). We first titrated the infectivity of the strains in HeLa cells to reach similar infection load in order to enable a cross-strain comparison in GI cells and obtained a similar infection rate for Ctr WT and Ctr Δpgp3 and a slightly higher rate for Ctr PF (S5A Fig). Next, we performed the infectivity assay in human GI cells derived from three different donors using proportionate bacterial loads. During primary infection in gastric cells, Ctr Δpgp3 and Ctr PF showed significantly reduced infectivity (approx. 2.3-fold and 2.9-fold lower, respectively) compared to Ctr WT (Fig 4B) and a similar trend was observed in small and large intestinal cells (Fig 4C and 4D). To assess chlamydial development and fitness, equal amounts of cell lysates were transferred onto freshly seeded HeLa cells 48 hours p.i. and analyzed 24 hours p.i. (here referred to as progeny infection). Ctr Δpgp3 from gastric cells formed significantly fewer (2.8-fold fewer) infective progeny compared to Ctr WT (Fig 4E) and a similar trend was observed in intestinal cells (Fig 4F and 4G). Ctr WT and Ctr Δpgp3 grown in HeLa cells formed equal amount of progeny, and Ctr PF formed significantly fewer (approx. 1.8-fold fewer) progeny compared to Ctr WT and Ctr Δpgp3 despite the initially higher load in primary infection (S5B Fig).
(A) Schematic representation of the primary and progeny infection assays. To compare the infectivity of Ctr WT, Ctr Δpgp3 and Ctr PF strains in gastric (B), small intestinal (C) and large intestinal (D) cells, subconfluent monolayers of the cells were infected using MOI of 5. 24 hours p.i. the cells were fixed, stained and the infection rate was determined by quantifying the number of inclusions and host cell nuclei in 14 fields of view per sample by automated microscopy. To assess the infectivity of the chlamydial progeny, the infected cells were lysed 48 hours p.i. and freshly seeded HeLa cells were infected with an aliquot of the lysates. 24 hours p.i. the HeLa cells were fixed, stained and the infection rates for the chlamydial progenies from gastric (E), small intestinal (F) and large intestinal (G) cells were determined in 14 fields of view per sample by automated microscopy. All graphs represent the mean ± SD from three independent experiments. Statistical significance was determined by one-way ANOVA (ns = not significant, **P<0.01, ***P<0.001, ****P<0.0001). The data point numbers and shapes below the graphs refer to the donor IDs used in the experiments. Fig 4A was prepared in Biorender.com.
To confirm the results by measuring another parameter for chlamydial development, we also determined the average size of the inclusions during primary infection. Interestingly, although Ctr WT and Ctr Δpgp3 formed inclusions of similar size in HeLa cells (S5C Fig), in gastric cells Ctr Δpgp3 formed significantly smaller inclusions compared to Ctr WT (Fig 5A) and a similar trend was observed in intestinal cells (Fig 5B and 5C). Taken together, these results suggest that the lack of the Pgp3 reduces the infectivity of C. trachomatis in human primary gastric and possibly also in intestinal epithelial cells.
Subconfluent monolayers of gastric (A), small intestinal (B) and large intestinal (C) cells were infected with Ctr WT, Ctr Δpgp3 and Ctr PF strains at MOI of 5. 24 hours p.i. the cells were fixed, stained and the average size of the inclusions was determined by automated microscopy in 14 fields of view per sample. All graphs represent the mean ± SD from three independent experiments. Statistical significance was determined by one-way ANOVA (ns = not significant, *P<0.05, **P<0.01, ***P<0.001). The data point numbers and shapes below the graphs refer to the donor IDs used in the experiments.
Human GI cells harbour aberrant developmental forms of C. trachomatis
Human intestinal tissue is hypothesized to represent a potential niche for persistent chlamydial infection, which is frequently characterized by the appearance of so-called aberrant bodies (ABs), irregular particles much larger than RBs [23,33]. We therefore characterized chlamydial inclusions in human GI cells at the ultrastructural level by using transmission electron microscopy (TEM). Gastric and intestinal cells derived from 3 different donors were infected with C. trachomatis and 40 hours p.i. processed for TEM. Surprisingly, we found a mixed population of normal (Fig 6A) and aberrant (Fig 6B) developmental forms of inclusions in both gastric and intestinal cells. Morphological evaluation of the obtained micrographs revealed different morphologies of aberrant inclusions, some of them containing exclusively enlarged ABs and some others harbouring also dividing forms of RBs or even EBs. In contrast to GI cells, no ABs could be detected in infected HeLa cells. This indicates that gastric and intestinal cells could harbour ABs and thus potentially may serve as a reservoir for persistent infection.
Subconfluent monolayers of gastric, small and large intestinal epithelial cells derived from three different donors were pre-treated with EGTA and infected with C. trachomatis at MOI of 5 and HeLa cells were infected at MOI of 0.5. 40 hours p.i. the cells were fixed and processed for TEM. Shown are representative images of the normal (A) and aberrant-like (B) inclusions found in the respective cells. Triangles indicate EBs (blue), RBs (yellow) and ABs (red). Scale bar: 2 μm.
Discussion
Increasing evidence suggests that the mucosa of the GI tract provides a niche for persistent C. trachomatis infections in the human body and can potentially cause repeated infections in other tissues, including the genital tract [23]. Nevertheless, there is only a limited number of studies on the pathogenesis of C. trachomatis in human GI cells and most of the knowledge comes from murine infection models. So far, C. trachomatis GI infection has been studied using cancer cell line models, such as human enteroendocrine LCC-18 and CNDT-2 cells [34], Caco-2 and COLO-205 colon carcinoma cells [35]. In the present study, we modelled C. trachomatis infection in human primary gastric and intestinal epithelial cells.
Our results based on 3D and 2D infection models indicate that both gastric and intestinal cells can support the chlamydial development. However, attachment of Chlamydia to the basolateral membrane is necessary for establishing an infection. In the GI monolayer model, the tightly arranged cell architecture renders the cells resistant to infection from the apical surface, whereas the infection from the basolateral surface is highly efficient (Fig 3B). In line with this, in subconfluent GI monolayers C. trachomatis infects the marginally located cells, even after completion of several chlamydial developmental cycles (Fig 1C). We assume this is due to the localization of the chlamydial entry receptors on the basolateral, but not on the apical surface of the cells. Consequently, only the cells on the edges of the cell patches allow Chlamydia to access the basolateral side, while other cells are engaged in junctional complexes. This theory is substantiated by the observation that disruption of the cell-cell contacts by the Ca2+ chelating agent EGTA increases the number of infected cells and renders the infection pattern more random (Fig 2B and 2C). In polarized epithelial cells tight and adherens junctions shield the basolaterally expressed integrins [30], which prompted us to assess the role of the known chlamydial entry receptor integrin β1 as a potential candidate receptor. Blocking of the receptor on the basolateral surface of the gastric cells led to a decreased infection rate, confirming its involvement in the infection (Fig 3D and 3E). Recently, it has been demonstrated that EphA2, another chlamydial entry receptor, is strictly localized at cell-cell junctions in primary gastric epithelial cells [15]. Thus, further studies are needed to evaluate the involvement of EphA2 and other basolaterally positioned receptors in the C. trachomatis infection of human GI cells.
To check if the basolateral route of chlamydial infection might take place in other columnar epithelial cell models as well, we modelled the infection in human primary fallopian tube cells. Although we could detect inclusions in apically infected samples, the basolateral infection resulted in a significantly higher infection rate (S4B and S4C Fig). This might explain the low infection rate (<5%) previously reported in a similar apically infected fallopian tube model [36]. However, it is to note that in contrast to GI cells, the culture of human fallopian tube cells on 3 μm pore-size membranes (a technical requirement imposed by the experimental setup) compromises their ability to polarize and hinders the comparison with the GI model.
The crucial role of the plasmid for the successful colonization of the GI tract by Chlamydia has been demonstrated in the mouse model of infection [26]. The importance of the plasmid is further evidenced by the fact that naturally occurring plasmid-free clinical isolates are very rare [37,38]. Following oral inoculation, Chlamydia overcome several GI barriers in mice in order to reach and colonize the large intestine, and plasmid lacking mutants, in particular Pgp3-mutants, are defective in colonizing the upper GI tract of the mice [26,32]. It is speculated that Chlamydia use plasmid-encoded factors to colonize the upper GI tract, whereas chromosome-encoded factors are more important for colonization of the lower GI tract [26]. We compared the infectivity of the wild-type, Pgp3-deficient and plasmid-free C. trachomatis in human GI cells to check if the plasmid-associated infectivity defects reported in vivo could be reproduced in a human system in vitro. In order to obtain high-throughput readouts for the infectivity assay, we used the Operetta (Perkin-Elmer) automated plate imaging system for the analysis, which required that the human primary GI cells be cultured in μ-plates (Ibidi) optimized for inverted fluorescence-based imaging. Since these plates are not suitable for infection from the basal surface of the cells, the GI cells had to be grown as subconfluent monolayers to make them susceptible to C. trachomatis infection. The analysis revealed that compared to the wild-type, Ctr Δpgp3 formed significantly fewer and smaller inclusions in human gastric cells and a similar trend was observed in intestinal cells (Figs 4 and 5). In subconfluent GI monolayers, the number and the size of the cell islands formed significantly affect the efficiency of C. trachomatis infection. Compared to the gastric cells, the generation of subconfluent intestinal monolayers of identical confluency is technically more challenging and likely underlies the higher variability of the data points and the resulting lack of the significance in the infected intestinal cells.
Pgp3 is essential for C. muridarum to colonize the upper GI tract of the mice, allowing Chlamydia to resist the gastric acid in the stomach and evade from the CD4+ T lymphocyte-mediated immunity in the small intestine [26,39,40]. However, our epithelial model does not contain immune cells nor is gastric acid produced, because the appropriate cells (parietal cells) are absent in the system. In addition, it has been reported that Pgp3 can bind to the human antimicrobial peptide LL-37 (cathelicidin) to neutralize its anti-chlamydial activity and also inhibit the LL-37-mediated proinflammatory immune responses in human epithelial cells [41,42]. Human primary GI epithelial cells possess innate defense mechanisms and can initiate inflammatory responses against the invading pathogens [43]. It has also been previously reported that the antimicrobial peptides, including LL-37, are upregulated in these cells in response to infection [44–46]. Thus, the absence of the Pgp3 in the Ctr Δpgp3-infected cells may have rendered Chlamydia more susceptible to the cellular innate immune responses. Further studies are needed to clarify the specific role of Pgp3 during the infection of GI epithelial cells.
The infectivity assay also showed smaller inclusions and a growth defect for the plasmid-free Chlamydia in the infected GI cells, at levels comparable to Ctr Δpgp3 (Figs 4 and 5). However, plasmid-free Chlamydia also exhibit a growth defect in HeLa 229 cells (S5B Fig). Although the plasmid is not essential for the chlamydial growth in transformed cell lines, its absence has been shown to affect the attachment/uptake of Chlamydia and result in reduced infectivity in vitro [37,38,47]. Centrifugation can overcome the infectivity defect, however, we performed the infection experiments without centrifugation. This may explain the reduced infectivity of plasmid-free Chlamydia in HeLa cells (S5B Fig).
In search of signs of persistence, we performed electron microscopic analysis of infected GI cells, which revealed that gastric, small and large intestinal cells infected with C. trachomatis harbor not only normal developed inclusions, but also aberrant developmental forms, which have different morphologies (Fig 6). 2D monolayer cultures of primary GI cells are not monotypic and contain different cell types and we assume that possibly some cell types restrict, while some others permit chlamydial development. Interestingly, inclusions of similar morphology have been found in the intestine of the pigs naturally or experimentally infected with C. suis [48].
Taken together, our infection model replicated phenotypes predicted and expected for C. trachomatis human intestinal infection, like the occurrence of persistent infection, and therefore supported a role of the human GI tract as a potential niche for chlamydial infection. At the same time, our results in 2D monolayers imply that the healthy intact GI epithelium is resistant to luminal C. trachomatis infection. It is likely that prior events that compromise the epithelial barrier integrity and polarity, such as inflammation, epithelial-mesenchymal transition, malignant transformation, or mechanical microinjury of the intestinal mucosa, may be necessary for the establishment of infection. Notably, cellular junctions and polarity are frequently targeted by various bacterial and viral pathogens that have evolved mechanisms to manipulate the protective barrier function of the mucosal epithelium to facilitate their spread [49].
Intestinal microfold cells (M cells) are specialized cells found in the GI tract that possess high transcytotic activity and play a critical role in the immunosurveillance by transporting the antigens and pathogens from the gut lumen to the subepithelial immune cells. Exploitation of these cells as an entry portal is another important route of infection used by several bacterial and viral pathogens [50]. For example, Shigella flexneri invades the M cells and infects enterocytes from the basolateral pole after crossing the intestinal barrier [51]. Thus, the use of M cells may represent another hypothetical mechanism by which Chlamydia could gain access to the basolateral surface of the intestinal cells in vivo.
Our study highlights the importance of using physiologically relevant host models for modelling host-pathogen interactions. There are, however, clear limitations of our model like the absence of natural microbiota and a functional innate and adaptive immune system. The intestinal microbiota and immune system are important factors that protect the intestinal epithelium from pathogens [52]. Initial attempts have been undertaken to generate human intestinal models complemented with human microbiota and innate immune cells [53,54]. Although these systems still do not adequately resemble the natural human intestinal environment, they will be useful to further investigate the role of chlamydial infection in the human GI tract.
Methods
Ethics statement
This study was approved by the ethical committee of the University Hospital of Würzburg (approval 37/16, 36/16 and 261/20). Written consent was obtained from all donors.
Culture of organoids
Patient-derived gastric (corporal), small intestinal (jejunal), large intestinal (colonic) and fallopian tube organoid lines were retrieved from an established biobank and cultured in Matrigel drops (Corning, 356231) overlaid with the corresponding organoid culture medium in 24-well plates and kept in a humidified incubator at 37°C and 5% CO2. Advanced DMEM/F12 (Thermo Fisher Scientific, 12634028) supplemented with 10 mM HEPES (Thermo Fisher Scientific, 15630056) and 1% GlutaMAX (Thermo Fisher Scientific, 35050061) was used as a basal medium and the organoid-specific growth factors were added to it (full composition of the media is provided in S1 Table). Organoids were passaged every 7–14 days at a ratio 1:2 to 1:10 depending on the donor and the medium was changed every 2–3 days. During the first two days of culture after splitting, media were supplemented with 10 μM ROCK inhibitor (AbMole Bioscience, Y-27632).
Culture of organoid-derived 2D monolayers
2D monolayers in the microwell plates were generated according to previously established protocols [55]: organoids were dissociated into single cells using TrypLE (Thermo Fisher Scientific, 12604013) at 37°C, seeded into the microwell plate and grown in corresponding organoid culture medium at 37°C and 5% CO2. To generate monolayers on cell culture inserts (Sigma Aldrich, PITP01250), dissociated cells were seeded onto the membrane and grown in corresponding organoid culture medium till reaching confluency at 37°C and 5% CO2.
Culture of cell lines
Human cervix adenocarcinoma cells (HeLa 229, ATCC CCL-2.1) were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 72400054) supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524) and maintained in a humidified incubator at 37°C and 5% CO2.
C. trachomatis strains and cultivation
All C. trachomatis LGV strains used in the study originate from the C. trachomatis L2 (434/Bu, ATCC VR-902B). The GFP-expressing strain was generated by transforming C. trachomatis L2 with pGFP::SW2 plasmid as previously described [56]. The plasmid-free strain was generated using novobiocin treatment as previously described [37]. The Pgp3 deletion mutant was generated by transforming the plasmid-free C. trachomatis L2 with pGFP::SW2Δpgp3 plasmid. Genital C. trachomatis strain E (BOUR, ATCC, VR-348B) and C. trachomatis strain K (UW-31/Cx, ATCC, VR-887) were used in the study.
For stock preparation, the strains were propagated in HeLa 229 cells for 48 hours, after which the cells were lysed with glass beads and Chlamydia were separated by centrifugation at 2,000 x g for 10 min at 4°C. Chlamydia were afterwards pelleted by centrifugation at 30,000 x g for 30 min at 4°C and washed with 1x sucrose-phosphate-glutamic acid buffer (SPG). The bacterial pellet was re-suspended in 1x SPG buffer, aliquoted and stored at -80°C. For infectivity titration, HeLa 229 cells grown in 24-well microplates (Ibidi, 82426) were infected with different amounts of bacteria for 2 hours and 24 hours p.i. the cells were fixed and stained (details of the staining provided in Immunofluorescence). The number of chlamydial inclusions and host cell nuclei was measured and analyzed with Operetta automated microscopy system (Perkin-Elmer). The amount of the Chlamydia resulting in a Chlamydia inclusion to HeLa cell nuclei ratio of 0.5 was determined and the obtained infection rate was considered as a multiplicity of infection (MOI) of 0.5. Ten times more bacteria were used for the infection of primary epithelial cells (MOI relative to HeLa cells). The titration of the genital C. trachomatis strains E and K was conducted using a flow cytometer (Thermo Fisher Scientific, Attune NxT) following an adapted version of a previously published protocol [57].
Infection of the organoids
Organoids were infected following a published protocol [16]: briefly, mature organoids were released from Matrigel drops with cold basal medium and mechanically disrupted into small fragments. Fragmented organoids were centrifuged (300 x g for 5 min at 4°C) and the pellet was infected with GFP-expressing C. trachomatis L2 for 20 min at MOI of 5. After incubation, the pellet was re-suspended in fresh Matrigel and seeded in a microwell plate. The infection was monitored daily using phase-contrast and fluorescence microscopy (Leica DMI3000B).
Infection of the monolayers
For the 2D infections in microwell plates, HeLa and primary GI cells were seeded in 24-well microplates (Ibidi, 82426) and grown to 70% confluency. For the 2D long-term infections (Fig 1C), HeLa and GI cells were infected with C. trachomatis at MOI of 0.5 and after 2 hours of incubation, the medium of the plates was exchanged. Images were obtained with a phase-contrast and fluorescence microscope (Leica DMI3000B) every 24 hours and the medium change was performed as needed. For the 2D infectivity assays HeLa cells were infected at MOI of 0.5 and the primary GI cells were infected at MOI of 5. After 2 hours of infection, the medium of the plates was exchanged. To assess the primary infection, 24 hours p.i. the cells were fixed, stained and the number of inclusions and the host cell nuclei was quantified with Operetta automated microscopy system. To assess the infectivity (progeny infection), infected cells were lysed with glass beads 48 hours p.i. and dilutions of the supernatant were used to infect freshly seeded HeLa cells, which were fixed 24 hours p.i., stained and analyzed with Operetta automated microscopy system.
For infection assays in cell culture inserts, the bacterial inoculum resuspended in basal medium to a volume of 75 μl, was applied to the apical surface of cells in cell culture inserts or carefully added as a drop to the basolateral side on inverted inserts. After 2 hours of incubation, the inoculum was removed and the inserts were kept in the corresponding growth media. For receptor-blocking assays, the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 μg/ml, R&D Systems, MAB17781) for 1h at 37°C, followed by infection with C. trachomatis L2 at MOI of 0.5 in the presence of antibodies. After 2 hours of infection, the inoculum was removed and the cells were kept in corresponding growth media. The cells were fixed 24 hours p.i. and analyzed via confocoal microscopy. The number of chlamydial inclusions and host cell nuclei was determined using Fiji.
To induce disruption of the cell-cell junctions, cells were treated with 4 mM EGTA (Sigma Aldrich, E4378) for the specified amount of time at 37°C prior to infection.
Immunofluorescence
To perform whole-mount immunofluorescence staining, at designated times, the cells were washed with phosphate buffered saline (PBS, Thermo Fisher Scientific, 14190169) and fixed with 4% paraformaldehyde (PFA, Morphisto, 11762.01000). After permeabilization with 0.2% Triton X-100 (Roth, 3051.4) in PBS, cells were blocked with 2% FCS or 1% bovine serum albumin (Roth, 8076.3) in PBS followed by incubation with primary antibodies diluted in blocking buffer. For immunohistological analysis, the PFA-fixed membranes of the cell culture inserts were cut out with a scalpel and subjected to dehydration in ascending series of water, ethanol (50%, 70%, 90%, 96%), isopropanol, xylene using automated tissue processor (Leica TP1020) and subsequently incubated in liquid paraffin. The membranes were cut in half and embedded in a mold using the paraffin embedding station (Medite, TES99). 5 μm sections were cut using a sliding microtome (Leica SM2010 R). For immunostaining, the paraffin-embedded sections were deparaffinized and rehydrated in descending series of xylene, ethanol and water, treated with antigen-retrieval solution (Dako, S1700) and blocked in 1% bovine serum albumin in PBS followed by incubation with primary antibodies diluted in blocking buffer.
Following primary antibodies were used in the study: anti-HSP60 (Santa Cruz, sc-57840), anti-MOMP (Biozol, NAC-MAB12270-100), anti-E-cadherin (Becton Dickinson, 560064; Proteintech, 20874-1-AP), anti-integrin β1 (Santa Cruz, sc-59829), anti-Ki67 (Cell Signaling, 9129S). Alexa Fluor (Thermo Fisher Scientific) or Cy (Dianova) conjugated secondary antibodies were used. Actin filaments were stained with Phalloidin (MoBiTec, MFP-D555-33) and the DNA with DAPI (Sigma Aldrich, D9542). If necessary, the samples were mounted with Mowiol (Roth, 0713.2). Images were collected using a confocal microscope (Leica, TCS SP5) or Operetta automated microscopy system (Perkin-Elmer).
Live cell imaging
Gastric and HeLa cells were seeded in 8-well chamber μ-slides (ibidi, 80826) and grown in corresponding media untill reaching 70% confluency. Prior to imaging cells were incubated with 2.5 μM CellTracker Green (Thermo Fisher Scientific/Invitrogen, C2925) fluorescent probe for 30 min at 37°C and subsequently washed with PBS. Time-lapse imaging was performed on a Leica TCS SP5 confocal microscope with a 40x oil immersion objective (Leica HC PL APO CS2, NA = 1.3) 5 min after addition of 4 mM EGTA. During the imaging cells were incubated at 37°C and 5% CO2 using a live-cell incubation chamber (Life Imaging Systems) and images were recorded in 1 min time intervals in 8-bit mode at a resolution of 1024x1024. The LAS AF software (Leica) was used for setting adjustment and image acquisition, and Fiji for image-processing [58].
Transmission electron microscopy
Primary GI and HeLa cells were cultured in 24-well microplates (Ibidi, 82426) as subconfluent monolayers and infected with wild-type C. trachomatis L2 at MOI of 5 and 0.5, respectively. Prior to infection, the GI cells were pre-treated with 4 mM EGTA for 30 min. 40 hours p.i., cells were washed with PBS, fixed with 2.5% glutaraldehyde (Sigma) for 30 min at room temperature, washed with 50 mM Cacodylate buffer (Roth), incubated in OsO4/Cacodylate buffer for 1 hour and in 0.5% Uranylacetat overnight. Dehydrated samples were embedded in EPON and cut. Images were taken with JEOL JEM-1400 Flash microscope.
Statistical analysis
The experimental data were analyzed using Graphpad Prism 9.3.1 software. For the analysis at least three independent experiments were used, unless otherwise indicated. Statistical significance was determined using unpaired Student’s t-test between two groups and one-way or two-way ANOVA between multiple groups. Data are shown as mean ± SD. A P value of less than 0.05 represented a statistically significant difference.
Supporting information
S1 Fig. The morphology of uninfected GI organoids and monolayers.
Representative phase-contrast images of the human gastric (corporal), small intestinal (jejunal), large intestinal (colonic) organoids in a Matrigel drop and the subconfluent 2D monolayers derived from respective organoid cultures in microwell plates. Scale bar: 100 μm.
https://doi.org/10.1371/journal.ppat.1012144.s001
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S2 Fig. C. trachomatis infects Ki67-positive and negative gastric cells.
(A) Subconfluent gastric cells in microwell plates were infected with GFP-expressing C. trachomatis (MOI of 5) and 24 hours p.i. fixed, stained and subjected to confocal microscopy. Representative fluorescence microscopic images of three independent experiments show the localization of Ki67-positive cells in uninfected and infected samples (blue: DAPI, green: C. trachomatis, grey: actin, magenta: Ki67). Scale bar: 50 μm. (B) The percentage of the chlamydial inclusions residing in Ki67-positive or negative cells was determined by manually quantifying inclusions in five fields of view per sample. Data represent the mean ± SD from three independent experiments. Statistical analysis was performed by unpaired t-test (ns = not significant).
https://doi.org/10.1371/journal.ppat.1012144.s002
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S3 Fig. The effect of EGTA treatment on the morphology and infection pattern of intestinal cells.
(A) Small and large intestinal epithelial cells grown as subconfluent monolayers were treated with 4 mM EGTA for 30 min at 37°C or left untreated. Phase-contrast images show the changes in the morphology of the cells upon treatment. Scale bar: 100 μm. (B) Intestinal cells pre-treated with 4 mM EGTA for 30 min or left untreated, were infected with GFP-expressing C. trachomatis (MOI of 5). 24 hours p.i. the cells were fixed, stained and subjected to confocal microscopy (blue: DAPI, green: C. trachomatis, red: Actin). Scale bar: 100 μm.
https://doi.org/10.1371/journal.ppat.1012144.s003
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S4 Fig. C. trachomatis apical versus basolateral infection in human gastric and fallopian tube cells.
(A) Representative confocal microscopy image showing the results of apical (top image) and basolateral (bottom image) C. trachomatis infections in the cross-sections of polarized human primary gastric cells. Human primary gastric cells cultured in cell culture inserts were infected with C. trachomatis (MOI of 5) from the apical or basolateral surface and after 2 hours of incubation the inoculum was removed, and the cells were kept in fresh organoid medium. 24 hours p.i. the cells were washed and fixed with PFA. 5 μm histological cross-sections of the paraffin-embedded samples were used for the immunofluorescence analysis (blue: DAPI, green: C. trachomatis, red: E-cadherin). The fluorescence channels were merged with the bright field channel to visualize cell orientation. Scale bar: 20 μm. (B) Organoid-derived human primary fallopian tube cells grown on cell culture inserts were infected with GFP-expressing C. trachomatis (MOI of 5) from apical or basolateral surface․ After 2 hours of incubation, the inoculum was removed and the cells were kept in the fresh organoid medium. 24 hours p.i. the cells were fixed, stained and subjected to confocal microscopy. Shown are representative confocal fluorescence Z-stack images of four independent experiments (blue: DAPI, green: C. trachomatis). Scale bar: 50 μm. (C) The number of chlamydial inclusions and host cell nuclei in (B) was quantified in at least 4 fields of view per sample using Fiji. Data represent the mean ± SD from four independent experiments. Statistical analysis was performed by unpaired t-test (*P<0.05). The data point numbers and shapes below the graphs refer to the donor IDs used in the experiments. (D) Human primary gastric cells grown on cell culture inserts were infected with C. trachomatis serovar E (6.5 x 107 chlamydial particles) and serovar K (5.5 x 107 chlamydial particles) from the apical or basolateral surface and after 2 hours of incubation the inoculum was removed, and the cells were kept in fresh organoid medium. 24 hours p.i. the cells were fixed, stained and subjected to confocal microscopy. Shown are representative confocal fluorescence Z-stack images of at least three independent experiments (blue: DAPI, green: C. trachomatis). Scale bar: 50 μm. (E) The number of chlamydial inclusions and host cell nuclei in (D) was quantified in at least 4 fields of view per sample using Fiji. Data represent the mean ± SD of three independent experiments. Statistical analysis was performed by unpaired t-test (*P<0.05, **P<0.01).
https://doi.org/10.1371/journal.ppat.1012144.s004
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S5 Fig. Infection of the Pgp3-deficient and plasmid-free C. trachomatis in HeLa cells.
(A) Primary infection of HeLa cells infected with Ctr WT, Ctr Δpgp3 and Ctr PF. Subconfluent monolayers of HeLa cells were infected with the chlamydial derivatives at MOI of 0.5. 24 hours p.i. the cells were fixed, stained and the infection rate was determined by quantifying the number of inclusions and host cell nuclei in 14 fields of view per sample by automated microscopy. To assess the infectivity of the chlamydial progeny (B), the infected HeLa cells were lysed 48 hours p.i. and freshly seeded HeLa cells were infected with an aliquot of the lysates. 24 hours p.i. the cells were fixed, stained and the infection rate was determined in 14 fields of view per sample by automated microscopy. (C) The average size of the inclusions during primary infection was determined by automated microscopy in 14 fields of view per sample. All graphs represent the mean ± SD from three independent experiments. Statistical significance was determined by one-way ANOVA (ns = not significant, *P<0.05, **P<0.01, ***P<0.001).
https://doi.org/10.1371/journal.ppat.1012144.s005
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S1 Table. Media composition for human gastric, intestinal and fallopian tube organoids.
CM: conditioned medium; EGF: epidermal growth factor; FGF-10: fibroblast growth factor-10; TGF-β: transforming growth factor-β; IGF-1: insulin-like growth factor I; FGF-2: fibroblast growth factor-basic.
https://doi.org/10.1371/journal.ppat.1012144.s006
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S1 Data. Original data: Excel file with values behind means and standard deviation used to build graphs.
https://doi.org/10.1371/journal.ppat.1012144.s007
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Acknowledgments
We thank Claudia Gehrig-Höhn and Daniela Bunsen for assistance in sample preparation for TEM and imaging; Dr. Ursula Eilers and Dr. Christina Schülein-Völk for assistance in high-content automated imaging; Dr. Carmen Aguilar and Dr. Özge Kayisoglu for valuable advice. The Leica TCS SP5 microscope, Perkin Elmer Operetta High-Content Imaging System and JEOL JEM-1400 Flash microscopes were funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under project codes 116162193, 237502929 and 426173797, respectively.
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