SARS-CoV-2 M^pro, PL^pro, spike, and helicase proteins are toxic in S. cerevisiae

Six SARS-CoV-2 (Wuhan-Hu-1) NSPs and the structural genes were expressed from a high copy plasmid [18] to determine if any would result in growth effects (Figs 1A and S1A). We observed no marked growth phenotypes as determined by spot tests when M, E, N, NSP7, NSP8, or NSP12 were expressed (S1B Fig). In contrast, spot tests revealed nearly a complete absence of growth when cells expressed NSP3 (PL^pro), NSP5 (M^pro or 3CL^pro), NSP13 (Helicase), and spike (S1B Fig). Analysis of growth profiles of cells expressing PL^pro, M^pro, Helicase, and spike showed all four genes caused a reduction in growth. Previous reports have shown that expression of the SARS-CoV-1 PLpro reduces yeast growth [19]. M^pro and the Helicase were the most toxic conferring a ~70 to 80% reduction in total growth by 72 hours compared to cells carrying empty vector (Fig 1A and 1B). As M^pro is highly conserved between classes of coronavirus and a key drug target we focused our efforts on using the yeast system to study M^pro structure and function.

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Fig 1. M^pro confers a significant reduction in growth in yeast caused by decreases in a variety of cellular proteins.

A) The indicated SARS-CoV-2 genes regulated by a galactose inducible promoter were expressed in yeast and conferred growth defects compared to empty vector (EV). B) Bar graph shows the total growth of cultures after 72 hours normalized to EV. C) Galactose-induced (ga) expression of the catalytically inactive M^pro C145A mutant (C145A ga) does not confer a growth reduction compared to WT (M^pro ga). When grown in glucose (gl) all three strains grew equally well. D) Protein levels of the M^pro C145A mutant and wild-type M^pro (WT) are comparable. Shown are two biological replicates for each form of M^pro. Ratios of Flag:GAPDH signals relative to M^pro WT is shown at the bottom of each lane. E) Total protein lysates made from yeast expressing the wild-type M^pro (WT) or M^pro C145A mutant (MUT) were subjected to mass spectrometric analyses revealing 153 proteins were higher in abundance in the mutant relative to the wild-type. F) Gene Ontology (GO) analyses indicates an enrichment of proteins with functions in translation that are significantly reduced in the presence of M^pro versus M^pro C145A. Plots in A, B, C show averages from three biological replicates and error bars are standard deviations. (***) indicates differences (p<0.001)between EV and tested genes.

https://doi.org/10.1371/journal.ppat.1011592.g001

Growth defect conferred by M^pro expression depends on its catalytic activity and associated with decreased abundance in essential and non-essential yeast proteins

To determine if the growth reduction depended on M^pro proteolytic activity we constructed a catalytic mutant of M^pro by replacing the key cysteine at position 145 to an alanine, which prevents the initial protonation step needed for peptide bond hydrolysis [20,21]. Liquid growth assays showed that yeast expressing the M^pro C145A mutant grew as well as the yeast control carrying empty vector (Fig 1C). In contrast, all three strains grew similarly in glucose, which represses expression of M^pro (Fig 1C). Western analysis showed that yeast expressed similar levels of wild-type and M^pro C145A mutant (Fig 1D). These results demonstrate that the growth reduction observed in yeast expressing M^pro is dependent on its proteolytic activity.

Next, we measured the relative abundance of proteins in yeast expressing M^pro compared to yeast expressing the M^pro C145A catalytic mutant to determine the mechanism(s) that lead to loss of cell viability. Whole cell lysates were made from three independent cultures of cells expressing wild-type M^pro or the catalytic M^pro C145A mutant (Figs 1E and S2). The biological replicates were highly reproducible, and we observed peptides from 153 proteins (S1 Table) were significantly reduced in yeast expressing M^pro compared to the M^pro C145A mutant (Fig 1E.) Gene ontology analysis revealed an enrichment for genes with functions in translation (Fig 1F). In particular, multiple ribosomal proteins and translational regulators were reduced. There were a number of proteins that were significantly enriched in the M^pro catalytic mutant with functions in a variety of activities beyond translation (S1 Table) and several are known to be essential (S1 Table). Furthermore, approximately 16% (25/153) of reduced proteins contained at least one potential canonical M^pro site (LQ/S, G, A) (S2 Table) suggesting that some may be direct substrates of M^pro [22,23]. Another 36% (55/153) of reduced proteins carried non-canonical sites in which LQ was followed by any amino acid besides S, G, or A. These results show that expression of M^pro leads to decreases in a variety of proteins and eventual loss of translation that is likely the cause of the growth defects.

Nirmatrelvir restores growth to yeast expressing M^pro from multiple coronaviruses

Considering that the growth reduction conferred by M^pro activity is dependent on its proteolytic activity we tested if treating yeast with nirmatrelvir, would suppress the growth reduction. We tested nirmatrelvir at several concentrations and observed no cytotoxic effects (Fig 2B). Treating cells with increasing doses of nirmatrelvir led to a corresponding increase in growth (Fig 2A). At 100μM and 200μM of nirmatrelvir, growth was restored to similar levels as cells carrying empty vector (Fig 2A). As a qualitative measure to compare the effects of nirmatrelvir, we estimated the concentration of drug required to restore 50% of growth (RC₅₀) relative to that of untreated M^pro expressing cells. Based on this criterion we calculated RC₅₀ for nirmatrelvir to be 110.47 ± 4.76μM (Fig 2A and 2H). To determine if M^pro from other coronaviruses could be studied similarly, we tested the recent Omicron variant, M^pro P132H, which is currently the dominant form of M^pro, and M^pro from SARS-CoV-1 and Bat-CoV-HKU9. We observed that in all cases M^pro conferred a significant growth reduction (Figs 2C and S3). Nirmatrelvir has been reported to have broad M^pro specificity [7,9]. Consistent with this work, we observed that nirmatrelvir could restore growth in yeast expressing M^pro from all three forms of M^pro (Figs 2E, 2H, and S3).

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Fig 2. Yeast growth assays identify nirmatrelvir resistant M^pro mutants.

A) Total growth of cultures after 72 hours expressing M^pro in the presence of increasing doses of nirmatrelvir normalized to growth of yeast carrying empty vector (EV) are plotted. Growth is restored by nirmatrelvir in a dose dependent manner. B) No growth effects are observed in cells treated up to 200μM nirmatrelvir. C) Yeast expressing substitutions E166D and E166N grow as well as EV but E166R, P132H, and N142A results in significant growth reduction comparable to wild-type M^pro. D) Western analysis shows that mutants and wild-type M^pro are expressed at comparable levels. Ratios of Flag:GAPDH signals relative to M^pro WT is shown at the bottom of each lane. E—G) Cells expressing P132H and N142A remain sensitive to nirmatrelvir, indicated by growth recovery, but E166R appears to be resistant as there is a lack of growth even when treated with 200μM of nirmatrelvir. H) RC₅₀ measurements of each mutant in response to nirmatrelvir treatment. For all experiments, at least three biological and three technical replicates were performed. Error bars represent standard deviations. (*, p<0.01; ***, p<0.001) indicates differences compared to EV.

https://doi.org/10.1371/journal.ppat.1011592.g002

Characterization of potential nirmatrelvir resistant mutations in M^pro

We tested if growth of yeast expressing M^pro could be used as an approximation for M^pro activity. Thus, providing a system to rapidly determine the effects of mutations on M^pro activity and drug resistance. A variety of interactions (H-bonds, salt-bridges, van der waals) mediate binding between the catalytic site of M^pro and inhibitors [24–26]. While knowledge of the residues in contact with the inhibitor can inform predictions that may compromise inhibitor binding it is not obvious what amino acid substitutions would maintain M^pro activity toward substrate while compromising inhibitor interactions. With our yeast system we can easily test the effect of substitution mutations and rapidly determine if the mutations alter catalytic activity and sensitivity to inhibitor(s) by following growth phenotypes. To determine the feasibility of this approach we focused on E166, and N142 as these two residues form direct interactions with inhibitors and substrates [24,27].

We tested substitutions of E166 with three different amino acids that are yet to be dominant in the population. The following mutants predicted to be conserved (E166D), as the negative charge is maintained but with one less carbon in the side-chain; non-conserved (E166N), as asparagine is uncharged and has one less side chain carbon; and another non-conserved (E166R) substitution in which the arginine side chain is longer and positively charged.

We observed that all three substitution mutants were expressed at levels comparable to wild-type M^pro (Fig 2C). Expression of M^pro E166D and M^pro E166N did not cause a reduction in growth and grew as well as empty vector controls (Figs 2C, 2D and S4A). These results indicate that M^pro E166D and M^pro E166N may have defects in their enzymatic activities. However, the M^pro E166R mutant conferred a growth reduction that matched the wild-type M^pro, suggesting that its catalytic activity was intact (Figs 2C and S4B). While this manuscript was in preparation it was reported from in vitro SARS-CoV-2 evolution experiments that E166A and E166V substitutions conferred nirmatrelvir resistance [14,16,17]. Thus, we tested these two mutants in yeast and observed that both mutants were expressed at comparable levels and conferred a growth reduction similar to wild-type M^pro (S5A and S5B Fig).

Next, we challenged cells expressing M^pro E166R, E166A, and E166V with increasing concentrations of nirmatrelvir (25μM, 50μM, 100μM, or 200μM) and observed no significant improvement in growth remaining nearly identical to the untreated cultures (Figs 2F, S4B, S5C and S5D). Based on these experiments, the RC₅₀ for nirmatrelvir is >200μM compared to wild-type M^pro (Figs 2H, S5C and S5D). These results suggest that the E166R, E166A, and E166V mutations confer resistance to nirmatrelvir.

We constructed a substitution at position N142, which is known to contribute to inhibitor and substrate binding [7] and is yet to be present in the population. To inform on the specific substitution to make we used a distantly related M^pro from the gamma-coronavirus, IBV, which is conserved but displays slight divergence from SARS-CoV-2 M^pro [9]. We replaced N142 with alanine (M^pro N142A), as alanine is found in the IBV M^pro at the homologous site [28]. We observed M^pro N142A was expressed at levels comparable to wild-type and conferred a similar reduction in growth (Fig 2C and S4C) showing that it remained active. The RC₅₀ for nirmatrelvir increased modestly by ~1.5-fold (Fig 2G and 2H). These results show that the substitution mutants E166A, E166V, and E166R lead to nirmatrelvir resistance while N142A results in little difference from wild-type and E166N and E166D cause a significant loss in activity.

In vitro protease assays confirm that M^pro E166R, E166A, E166V are highly resistant to nirmatrelvir

To determine how well yeast growth assays correlated with standard enzymatic assays we directly measured proteolyitc activity using recombinant M^pro WT, E166N, E166D, E166R, E166A, E166V, and N142A. Compared to wild-type M^pro, the catalytic efficiencies (k_cat/K_m) of M^pro E166R, E166A, and E166V were decreased by ~16-fold, ~8-fold, and ~10-fold, respectively (Figs 3A and S6A). In contrast, E166N and E166D displayed severe reductions in catalytic efficiencies of ~84- and ~74-fold compared to WT, respectively (Fig 3A). On the other hand, M^pro N142A displayed a slight increase in catalytic efficiency of ~1.4-fold compared to WT (Fig 3A). To determine the response of the mutants to inhibitors we performed IC50 and Ki measurements. We observed for M^pro E166R, E166A, and E166V, increases in IC50’s of ~143-, ~6-, >300-fold for nirmatrelvir compared to WT, respectively (Figs 3B, 3C, S6B and S6C). On the other hand, M^pro N142A, only minor increases were observed in IC₅₀’s of ~1.4-fold for nirmatrelvir (Fig 3B). The K_i values for the inhibitors in assays with M^pro E166R, E166A, and E166V were increased by >1600-, >47-, and >5000-fold for nirmatrelvir, ~423-,~27-, ~790-fold for PF-0085231, and ~37, ~9-, ~38-fold for GC-376, respectively. (Figs 3C and S6C). Nearly no difference in Ki values was observed between wild-type M^pro and M^pro N142A, ~1.2-fold for nirmatrelvir, ~0.9-fold for PF-0085231, ~1.5-fold for GC-376) (Fig 3C). The enzymatic assays confirm the results from the yeast assays showing that M^pro E166R, E166A, and E166V are resistant to nirmatrelvir and also show that there is cross-resistance to PF-0085231 and GC-376 (Figs 3B, 3C and S6C). Similarly, results from yeast assays of M^pro N142A mutant appears to correspond well to the in vitro assays as both show minor to no increases in resistance (Fig 3B and 3C). Furthermore, the M^pro E166N and E166D mutants, which is not predicted to be catalytically active from the yeast assay, displayed >70-fold decreases in activity compared to wild-type in the in vitro assays. This result is completely consistent with observing no growth reduction when expressed in yeast. Taken together there is good correlation between the enzyme and yeast assays.

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Fig 3. Enzymatic assays demonstrate that E166N and E166D have severe defects in catalytic activity and M^pro E166R is highly resistant to nirmatrelvir.

A) Michaelis–Menten plot of M^pro and its mutants with various concentrations of FRET substrate. The K_m, V_max, k_cat, and k_cat/K_m values are shown in the table. B) The IC₅₀ plots of nirmatrelvir, PF-00835231. and GC-376 against M^pro WT, N142A, E166R, E166N, and E166D. C) K_i plots of nirmatrelvir, GC-376, and PF-00835231 against M^pro, M^pro E166R, and M^pro N142A.

https://doi.org/10.1371/journal.ppat.1011592.g003

Crystal structure of M^pro E166R reveals a loss of interactions leading to drug resistance

We were particularly interested in how replacing glutamate at position 166 with arginine led to a >1000-fold increase in resistance to nirmatrelvir while a substitution with asparagine led to an 83.5-fold decrease in enzymatic activity even though E166 is not known to be directly involved in catalysis. Toward addressing both questions, we solved the crystal structure of apo M^pro E166N and the complex structure of M^pro E166R with GC-376 at 2.3 and 2.1 Å resolution, respectively (Fig 4). Both proteins were crystallized in the C2 space group with one M^pro molecule per asymmetric unit, and the biological dimer can be generated through crystallographic symmetry. The conformations of the protein and ligand are therefore exactly the same between the two protomers of the dimer in these structures. Our efforts to obtain nirmatrelvir complex failed due to the relatively low compound solubility and the reduced binding for the mutant. But both nirmatrelvir and GC-376 have the same pyrrolidone side chain placed in the S1 site where E166 is located. In previous WT complex structures (PDB codes, 7RFW (nirmatrelvir), 6WTT(GC-376)), the protein and the pyrrolidone side chain adopted identical conformations in this area. The GC-376 complex can thus offer important insights into how E166 mutations may impact the binding of ligands with similar chemical structures, including nirmatrelvir.

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Fig 4. Crystal structures reveals structural basis for E166R resistance and E166N inactivity.

A) Apo M^pro WT (white, PDB 7JP1) aligned with apo M^pro E166N (green, PDB 8DDI). B) M^pro WT GC-376 complex (white, PDB 6WTT) aligned with M^pro E166R GC-376 complex (magenta, PDB 8DDM). WT hydrogen bonds are shown as black dashes, and mutant hydrogen bonds are shown as red dashes. GC-376 is shown in white for the WT structure and cyan for the mutant structure. Mutations are indicated with red text. Ser1 from an adjacent protomer is indicated with orange text.

https://doi.org/10.1371/journal.ppat.1011592.g004

In the M^pro E166N mutant structure, N166 forms a hydrogen bond (HB) with H163, an interaction not observed between E166 and H163 in the wild-type M^pro structure (Fig 4A). This new HB prevents H163 from hydrogen bonding with the glutamine side chain of the substrate, an interaction crucial to substrate binding. The binding of the substrate would therefore require N166 to adopt a different conformation, breaking the HB with H163 and increasing the energetic cost. These observations are consistent with the drastic decrease of activity in the E166N mutant and lack of toxicity when expressed in yeast (Fig 2C) bringing to light how residues outside of the catalytic core can influence substrate binding.

In contrast, the longer and positively charged R166 side chain in the M^pro E166R mutant does not interact with H163, but rather extends into the solvent (Fig 4B). Therefore, the S1 site is open for substrate binding. However, the E166R mutation does affect ligand binding in several aspects. The negatively charged E166 side chain forms two crucial HBs, one with the N-terminus of the neighboring M^pro protomer in the biological dimer, and the other with the pyrrolidone side chain of inhibitors (in both nirmatrelvir and GC-376) or with the glutamine side chain of the substrate as described above. The E166R mutation would abolish this direct HB with the substrate or inhibitor, resulting in the pyrrolidone ring of GC-376 forming an alternative weak HB with F140 backbone carbonyl group (3.1 Å in length) in the mutant complex structure (Fig 4B). In addition, the N-terminus of the enzyme interacts with both E166 and the backbone carbonyl group of F140, and plays an important role in maintaining the structural stability of the enzyme active site. The E166R mutation eliminates the salt bridge with the N-terminus of the adjacent protomer, and further introduces electrostatic repulsion leading to small yet significant changes in the N-terminus conformation. Consequently, the distance between the N-terminal amine group and the F140 carbonyl group increased from 2.6 Å in the WT to 3.7 Å in the M^pro E166R mutant, diminishing the HB. This in turn may destabilize the loop that F140 resides on and also contains other important structural features involved in enzyme catalysis and ligand binding, including the backbone amide groups of Gly143 and Ser144 that form part of the oxyanion hole to stabilize the reaction transition state. This loop also contains the peptide bond between Leu141 and Asn142 that interacts with the two extra carbon atoms of the inhibitor pyrrolidone ring, but not with the substrate glutamine side chain. Destabilization of the region near F140 may increase the entropic cost of binding to the rigid pyrrolidone ring of nirmatrelvir and GC-376, more than the smaller and more flexible substrate glutamine side chain. For similar entropic reasons, the HB between the pyrrolidone ring and E166 might contribute more to inhibitor binding than that between the more flexible glutamine side chain and E166 (Fig 4B). Consequently, the E166R mutation may have a stronger effect on binding to inhibitors such as nirmatrelvir versus substrate.

Strains, media, and chemicals

All yeast strains carried a pdr5::G418 deletion in the BY4741 background (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0). Yeast were grown in liquid synthetic complete (SC) media (0.17% yeast nitrogen base, 0.5% ammonium sulfate, amino acid mix with appropriate drop out as noted, 2% glucose) or on solid SC media containing 2% agar at 30°C. Media and reagents for culturing yeast were from United States Biological (Salem, MA). M^pro and PL^pro inhibitors were from MedChemExpress (Monmouth Junction, NJ) and Selleck Chemicals (Houston, TX). All other chemicals were from Sigma Aldrich (St. Louis, MO) or VWR (Radnor, PA).

Expression of SARS-CoV-2 genes in yeast and mutagenesis

The indicated SARS-CoV-2 genes were codon optimized for yeast, tagged at the 3’ with a 3X-Flag epitope, carried on high copy plasmids and genes were under the control of the Gal1 promoter (see S3 Table). Site directed mutagenesis was performed using In-Fusion Cloning Kit (Takara). Primers used for mutagenesis can be found in S2 Table. The M^pro gene was sequenced to confirm that mutations were incorporated successfully.

Yeast transformation

A single yeast colony was used to inoculate 5ml liquid YPD (1% yeast extract, 1% yeast bacto-peptone, 2% glucose) and grown overnight at 30°C. The next day cells were washed and resuspended in 1ml lithium acetate/TE solution (100 mM lithium acetate, 10 mM Tris-HCl, 1 mM EDTA, pH 7.5). Cells were aliquoted (60 μl) into microcentrifuge tubes, followed by the addition of denatured salmon sperm DNA (50μg), 0.2μg of plasmid, 1ml polyethylene glycol (PEG) lithium acetate solution (40% (w/v) PEG 4000, 100 mM lithium acetate, 10 mM Tris-HCl, 1 mM EDTA, pH 7.5), and incubated for 45min at 30°C. This was followed by a 20min incubation at 42° and chilled for 2min on ice. Cells were washed and resuspended in 100μl H₂O and plated on selective SC agar plates, incubated for ~3 days at 30°C.

Protein extraction and western analysis

Cells were grown overnight in 5 ml SC-Ura, 2% raffinose at 30°C. The next day, fresh cultures were started with optical density OD₆₀₀ of 0.5 in 20 ml SC-Ura, 2% galactose at 30° for 6 hrs. Cells were then harvested, frozen in liquid nitrogen, and stored at −80°. For total protein extract, trichloroacetic acid was performed as described previously [43] and protein concentration was determined by BCA protein assay kit (Thermo scientific). Protein samples were separated by 4–12% gradient SDS-PAGE (GenScript) and blotted onto nitrocellulose or PVDF membranes. The following primary antibodies were used at 1:5000 dilution: anti-FLAG antibody (GenScript), and anti-GAPDH antibody (Proteintech). Secondary anti-mouse IgG HRP antibody was used at 1:7000 dilution (Promega). ChemiDoc (Bio-Rad) imaging system was used to detect chemiluminescence signals from western blots. Relative quantification of protein bands (α-Flag, α-GAPDH) from western blots was performed using Fiji [44].

Cell growth assays and RC₅₀ measurements

Cells were grown overnight in 5ml SC-Ura, 2% raffinose at 30°C. The next day, fresh cultures were started with an OD₆₀₀ of 0.1 in SC-Ura, 2% galactose, with or without inhibitors and transferred to to 96-well plates, incubated at 30°C on a a rotary shaker. Three independent transformants were used to test each form of M^pro. Each transformant was sampled three times for each assay. The plate was transferred to a Tecan Infinite 200 PRO plate reader, and OD₆₀₀ measurements were taken at 0, 24, 48 and 72 hours, with 5 flashes per well. Excel (Microsoft) was used to analyze the raw data. As a measure of inhibitory activity of nirmatrelvir we calculated a Recovery Concentration (RC₅₀). The slopes from the dose responses were calculated and used to estimate the concentration of inhibitor that improves growth to half-maximal relative to empty vector control after 72 hours of growth. As we only tested up to 200μM of nirmatrelvir, in cases where the RC₅₀ is beyond this concentration we indicate as >200μM.

Yeast proteomics

Cells were grown overnight in 10ml SC-Ura + 2% Raffinose media at 30°C. The next day, fresh cultures were started with OD₆₀₀ of 0.1 in 100ml SC-Ura + 2% galactose at 30°C for 6 hr. Cells were then harvested, frozen in liquid nitrogen, and stored at −80°. Protein extraction was performed as described previously [43] and protein concentration was determined using Pierce BCA protein assay kit (Thermo scientific).

To determine changes in the proteome associated with expression of M^pro versus M^pro C145A, in-solution tryptic digestion was performed as described [45] followed by desalting with a Pierce Peptide Desalting Spin Columns per the manufacturer’s protocol (ThermoFisher Scientific, cat no. 89852) and the peptides were dried by vacuum centrifugation. 600 ng of the final sample was analyzed by mass spectrometry. HPLC-ESI-MS/MS was performed as previously described [46]. In brief, MS/MS was performed in positive ion mode on a Thermo Scientific Orbitrap Fusion Lumos tribrid mass spectrometer fitted with an EASY-Spray Source (Thermo Scientific, San Jose, CA). NanoLC was performed using a Thermo Scientific UltiMate 3000 RSLCnano System with an EASY Spray C18 LC column (Thermo Scientific).

Tandem mass spectra were extracted from Xcalibur ‘RAW’ files and charge states were assigned using the ProteoWizard 2.1.x msConvert script using the default parameters(23). The fragment mass spectra were then searched against the Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker’s yeast) UniProt database (6067 entries) using Mascot (Matrix Science, London, UK; version 2.6) using the default probability cut-off score. Cross-correlation of Mascot search results with X! Tandem was accomplished with Scaffold (version Scaffold_4.8.7; Proteome Software, Portland, OR, USA). Probability assessment of peptide assignments and protein identifications were made through the use of Scaffold. Only peptides with ≥ 95% probability were considered. Progenesis QI for proteomics software (version 2.4, Nonlinear Dynamics Ltd., Newcastle upon Tyne, UK) was used to perform ion-intensity based label-free quantification similar to as previously described [46]. Principal component analysis and unbiased hierarchal clustering analysis (heat map) was performed in Perseus [47,48]. Gene ontology and KEGG pathway enrichment analysis was performed with DAVID [49]. Proteomics data has been deposited to ProteomeXchange. Project accession: PXD036325 and Project DOI:10.6019/PXD036325.

Recombinant M^pro and proteolytic activity assays

SARS-CoV-2 M^pro mutants were generated with QuikChange II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-M^pro as the template. The plasmid produces tag-free M^pro protein with no extra residue at either N- or C-terminus upon removal of the SUMO tag by SUMO protease digestion [21].

SARS-CoV-2 M^pro mutant proteins were expressed and purified as previously described [21,50] with minor modifications. Plasmids were transformed into E. coli BL21(DE3) competent cells and bacterial cultures overexpressing the target proteins were grown in LB (Luria-Bertani) medium containing 50 μg/mL of kanamycin at 37°C, and expression of the target protein was induced at an optical density (A600) of 0.6–0.8 by the addition of isopropyl β-d-1-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM. The cell culture was incubated at 18°C for 12–16 hrs. Bacterial cultures were harvested by centrifugation (8,000 ×g, 10 min, 4°C) and resuspended in lysis buffer containing 25 mM Tris (pH 8.0), 750 mM NaCl, 2 mM DTT, 0.5 mg/mL lysozyme, 0.5 mM phenylmethylsulfonyl fluoride (PMSF) and 0.02 mg/mL DNase I. Bacterial cells were lysed by alternating sonication (30% amplitude, 1 s on/1 s off) and homogenization using a tissue grinder. The lysed cell suspension was clarified by centrifugation (18,000 ×g, 30min, 4°C) and the supernatant was incubated with Ni-NTA resin for over 2 hrs at 4°C on a rotator. The Ni-NTA resin was thoroughly washed with 20 mM imidazole in washing buffer containing 50mM Tris (pH 8.0), 150mM NaCl, 2 mM DTT, and SUMO-M^pro protein was eluted with elution buffer containing 50 to 300mM imidazole, 50mM Tris (pH 8.0), 150mM NaCl, 2mM DTT. Fractions containing SUMO-M^pro proteins greater than 90% homogeneity were pooled and subjected to dialysis (two times) against a buffer containing 50mM Tris (pH 8.0), 150mM NaCl, 2mM DTT and 10% glycerol. SUMO protease digestion was carried out at 30°C for 1 hr to remove SUMO tag. Following digestion, SUMO Protease and SUMO tag were removed by Ni-NTA resin. The purified tag-free SARS-CoV-2 M^pro mutant proteins were fast frozen in liquid nitrogen and stored at -80°C.

For measurement of K_m/V_max of SARS-CoV-2 M^pro mutants, proteolytic reactions were carried out with optimized concentrations of the mutant proteins and a series of concentrations of FRET substrate, Dabcyl-KTSAVLQ/SGFRKME (Edans), ranging from 0 to 200 μM in 100μL of reaction buffer containing 20mM HEPES (pH 6.5), 120mM NaCl, 0.4mM EDTA, 4mM DTT, and 20% glycerol at 30°C in a BioTek Cytation 5 imaging reader (Agilent) with filters for excitation at 360/40 nm and emission at 460/40 nm. The SARS-CoV-2 M^pro FRET substrate used in this study is Dabcyl-KTSAVLQ/SGFRKME (Edans), which corresponds to the sequence between viral polypeptide NSP4-NSP5 junction from SARS-CoV-2 [21,51]. Reactions were monitored every 90s, and the initial velocity of the proteolytic activity was calculated by linear regression for the first 15min of the kinetic progress curves. The initial velocity was plotted against the FRET substrate concentrations using the classic Michaelis-Menten equation in Prism 8 software.

For IC₅₀ measurements, optimized concentrations of the mutant proteins were incubated with series concentrations of GC-376, PF-00835231 or nirmatrelvir (PF-07321332) in 100μL of reaction buffer at 30°C for 15 min, and the reaction was initiated by adding 10μM FRET substrate. The reaction was monitored for 1 hr, and the initial velocity was calculated for the first 15min by linear regression. The IC₅₀ was determined by plotting the initial velocity against various concentrations of the compounds using log (inhibitor) vs response-variable slope in Prism 8 software.

For K_i measurements, optimized concentrations of the mutant proteins were added to 20μM FRET substrate with various concentrations of GC-376, PF-00835231 or nirmatrelvir (PF-07321332) in 200μL of reaction buffer at 30°C to initiate the proteolytic reaction. The reaction was monitored for 2 hrs and the initial velocity was calculated for the first 90 min by linear regression. The K_i was calculated by plotting the initial velocity against various concentrations of the compounds using Morrison plot (tight binding) in Prism 8 software.

M^pro crystallization and structure determination

SARS-CoV-2 M^pro E166N/R was diluted to 5 mg/mL in protein buffer (50 mM Tris pH 7.0, 150 mM NaCl, 4 mM DTT). Protein for complex determination was incubated overnight at 4°C with 2mM GC-376. No precipitation was observed after incubation, and centrifugation was not necessary. Apo and complex crystals were grown using 1.5 μL:1.5 μL (protein:well solution) hanging drops and a well solution of 0.1 M MgCl₂, 20% PEG 3350, 10% 1,6-hexanediol, 0.1 M HEPES pH 7.5, and 0.1 M LiSO₄. E166N/R crystals grew overnight at 20°C. Crystals were cryoprotected using the well solution supplemented with 20% glycerol, and then flash-frozen in liquid nitrogen.

X-ray diffraction data (S4 Table) were collected at the Southeast Regional Collaborative Access Team (SER-CAT) 22-BM beamline at the Advanced Photon Source (APS) in Argonne, IL, and processed with HKL2000 and CCP4. PHASER was used for molecular replacement using a previously solved SARS-CoV-2 M^pro structure (PDB ID: 7LYH) as a reference model. The CCP4 suite, (23) Coot, (24) and the PDB REDO server (pdb-redo.eu) (25) were used to complete the model building and refinement. The PyMOL Molecular Graphics System (Schrödinger, LLC) was used to generate all images.