Figures
Molecular evidence for widespread drug resistance in canine hookworms across USA
Ancylostoma caninum is an important zoonotic gastrointestinal nematode of dogs and close relative of human hookworms. We identified a novel benzimidazole resistance mutation in β-tubulin - Q134H(CAA>CAT) - and used structural modelling and C. elegans CRISPR-Cas9 editing to show it confers benzimidazole resistance. Next-gen amplicon sequencing showed this novel mutation, and a previously described F167Y(TTC>TAC) mutation, to be widespread in A. caninum populations in pet dogs across the USA. This is the first study to show geographically widespread drug resistance in any gastrointestinal nematode of domestic dogs to date. Venkatesan et al 2023
Image Credit: Abhinaya Venkatesan
Citation: (2023) PLoS Pathogens Issue Image | Vol. 19(3) April 2023. PLoS Pathog 19(3): ev19.i03. https://doi.org/10.1371/image.ppat.v19.i03
Published: April 4, 2023
Copyright: © 2023 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ancylostoma caninum is an important zoonotic gastrointestinal nematode of dogs and close relative of human hookworms. We identified a novel benzimidazole resistance mutation in β-tubulin - Q134H(CAA>CAT) - and used structural modelling and C. elegans CRISPR-Cas9 editing to show it confers benzimidazole resistance. Next-gen amplicon sequencing showed this novel mutation, and a previously described F167Y(TTC>TAC) mutation, to be widespread in A. caninum populations in pet dogs across the USA. This is the first study to show geographically widespread drug resistance in any gastrointestinal nematode of domestic dogs to date. Venkatesan et al 2023
Image Credit: Abhinaya Venkatesan