Figures
Female sag(-)KI mosquito displaying 3xP3-specific GFP expression in the ommatidia of the compound eyes
Saglin is a salivary gland-specific protein that has already been shown to affect transmission of the malaria parasite Plasmodium. The protein is expressed only in the median lobe of the female salivary gland, particularly in the cells of the base, which comprise only about one-third of the lobe. To study the saglin promoter, its coding sequence was replaced with the 3xP3 promoter fused to GFP. The activity of the 3xP3 promoter is usually observed in the larval nervous system, but in some individuals leads to strong GFP expression in the adult ommatidia. Klug et al 2022
Image Credit: Dennis Klug
Citation: (2022) PLoS Pathogens Issue Image | Vol. 18(10) November 2022. PLoS Pathog 18(10): ev18.i10. https://doi.org/10.1371/image.ppat.v18.i10
Published: November 1, 2022
Copyright: © 2022 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Saglin is a salivary gland-specific protein that has already been shown to affect transmission of the malaria parasite Plasmodium. The protein is expressed only in the median lobe of the female salivary gland, particularly in the cells of the base, which comprise only about one-third of the lobe. To study the saglin promoter, its coding sequence was replaced with the 3xP3 promoter fused to GFP. The activity of the 3xP3 promoter is usually observed in the larval nervous system, but in some individuals leads to strong GFP expression in the adult ommatidia. Klug et al 2022
Image Credit: Dennis Klug