Figures
SIV-specific CAR/CXCR5 T cells localize to lymphoid follicles and contact SIV vRNA+ cells
Two days post-infusion, SIV-specific CAR/CXCR5 T-cells (red) and SIV (white) were detected with RNAScope in situ hybridization in lymphoid tissues from an SIV-infected macaque. Follicles were delineated by staining B cells (green) with anti-CD20 antibody. Cellular nuclei were stained (blue) with DAPI. The white haze within the follicle represents SIV free virions trapped in the FDC network, and punctate white staining represents SIV vRNA+ cells. Two CAR T cells are in direct contact with an SIV vRNA+ cell in the top right of the image providing evidence that CAR/CXCR5 T-cells specifically targeted SIV+ cells in lymphoid follicles. Pampusch et al.
Image Credit: Abdelaal HM, 2022
Citation: (2022) PLoS Pathogens Issue Image | Vol. 18(2) February 2022. PLoS Pathog 18(2): ev18.i02. https://doi.org/10.1371/image.ppat.v18.i02
Published: February 28, 2022
This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
Two days post-infusion, SIV-specific CAR/CXCR5 T-cells (red) and SIV (white) were detected with RNAScope in situ hybridization in lymphoid tissues from an SIV-infected macaque. Follicles were delineated by staining B cells (green) with anti-CD20 antibody. Cellular nuclei were stained (blue) with DAPI. The white haze within the follicle represents SIV free virions trapped in the FDC network, and punctate white staining represents SIV vRNA+ cells. Two CAR T cells are in direct contact with an SIV vRNA+ cell in the top right of the image providing evidence that CAR/CXCR5 T-cells specifically targeted SIV+ cells in lymphoid follicles. Pampusch et al.
Image Credit: Abdelaal HM, 2022