Figures
Toxoplasma recruits host ESCRT machinery during infection
T. gondii resides in a parasitophorous vacuole featuring vesicles that bud with a topology that is consistent with the involvement of host Endosomal Sorting Complexes Required for Transport (ESCRT) machinery. Accordingly, this super-resolution Structured Illumination Microscopy (SIM) image shows accumulation of a dominant negative mutant of the ESCRT-IV protein VPS4A fused to mCherry Red (shown in magenta) together with the ESCRT-III protein CHMP4B (green, stained with anti-CHMP4B) around the parasitophorous vacuole (cyan, stained with anti-TgGRA7) of T. gondii. The image is a maximum projection of 3D SIM images. Rivera-Cuevas et al.
Image Credit: Rivera-Cuevas Y, 2021
Citation: (2021) PLoS Pathogens Issue Image | Vol. 17(12) December 2021. PLoS Pathog 17(12): ev17.i12. https://doi.org/10.1371/image.ppat.v17.i12
Published: December 31, 2021
This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
T. gondii resides in a parasitophorous vacuole featuring vesicles that bud with a topology that is consistent with the involvement of host Endosomal Sorting Complexes Required for Transport (ESCRT) machinery. Accordingly, this super-resolution Structured Illumination Microscopy (SIM) image shows accumulation of a dominant negative mutant of the ESCRT-IV protein VPS4A fused to mCherry Red (shown in magenta) together with the ESCRT-III protein CHMP4B (green, stained with anti-CHMP4B) around the parasitophorous vacuole (cyan, stained with anti-TgGRA7) of T. gondii. The image is a maximum projection of 3D SIM images. Rivera-Cuevas et al.
Image Credit: Rivera-Cuevas Y, 2021