Figures
Evidence that HIV-1 Gag is recruited into small RNA granules that contain DDX6 but are largely distinct from P bodies
293T cells were mock transfected or transfected with an HIV-1 provirus encoding the Gag G2A mutant, which is arrested in the cytoplasm. Columns (left to right, with nuclei in blue): DDX6 IF reveals green foci seen at low gain/magnification representing P bodies; proximity ligation assay shows sites of DDX6-Gag G2A colocalization as red spots; and merge. Insets show cells at high gain/magnification. Angled arrows allow matching of P bodies between insets and low power fields. Dotted oval contains foci that are too small to be P bodies, and could represent smaller DDX6 foci that can be co-opted by Gag proteins. Lingappa et al.
Image Credit: Brook Barajas and Jaisri Lingappa, University of Washington
Citation: (2018) PLoS Pathogens Issue Image | Vol. 14(4) April 2018. PLoS Pathog 14(4): ev14.i04. https://doi.org/10.1371/image.ppat.v14.i04
Published: April 30, 2018
Copyright: © 2018 Barajas and Lingappa, University of Washington. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
293T cells were mock transfected or transfected with an HIV-1 provirus encoding the Gag G2A mutant, which is arrested in the cytoplasm. Columns (left to right, with nuclei in blue): DDX6 IF reveals green foci seen at low gain/magnification representing P bodies; proximity ligation assay shows sites of DDX6-Gag G2A colocalization as red spots; and merge. Insets show cells at high gain/magnification. Angled arrows allow matching of P bodies between insets and low power fields. Dotted oval contains foci that are too small to be P bodies, and could represent smaller DDX6 foci that can be co-opted by Gag proteins. Lingappa et al.
Image Credit: Brook Barajas and Jaisri Lingappa, University of Washington