Figures
An activated form of Rab11 is specifically targeted to intercellular junctions in Drosophila salivary glands.
Drosophila larval salivary glands are comprised of giant polyploid cells, making them an ideal system for sub-cellular distribution and co-localization studies. In this image, an activated and YFP-tagged form of Rab11 was expressed in salivary glands. Rab11 is critical for endocytic recycling, a process through which vesicles carrying junctional components like cadherins are brought near and fuse with the plasma membrane. Only the activated form of Rab11 displays this strong preference for intercellular junctions. The anthrax toxin edema factor (EF) blocks Rab11 function, which weakens the adherens junctions, and promotes edema during infection. Guichard et al.
Image Credit: Annabel Guichard and Ethan Bier, Section of Cell and Developmental Biology, UCSD
Citation: (2017) PLoS Pathogens Issue Image | Vol. 13(9) September 2017. PLoS Pathog 13(9): ev13.i09. https://doi.org/10.1371/image.ppat.v13.i09
Published: September 29, 2017
Copyright: © 2017 Guichard and Bier. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Drosophila larval salivary glands are comprised of giant polyploid cells, making them an ideal system for sub-cellular distribution and co-localization studies. In this image, an activated and YFP-tagged form of Rab11 was expressed in salivary glands. Rab11 is critical for endocytic recycling, a process through which vesicles carrying junctional components like cadherins are brought near and fuse with the plasma membrane. Only the activated form of Rab11 displays this strong preference for intercellular junctions. The anthrax toxin edema factor (EF) blocks Rab11 function, which weakens the adherens junctions, and promotes edema during infection. Guichard et al.
Image Credit: Annabel Guichard and Ethan Bier, Section of Cell and Developmental Biology, UCSD