Figures
The adherens junction protein PVRL4 (Nectin 4) is an epithelial cell receptor for measles virus.
Measles virus infects primary human airway epithelial cells and cell lines derived from lung, breast, and colon adenocarcinomas. Microarray analysis of genes expressed in cells susceptible and non-susceptible to measles virus revealed that PVRL4 (Nectin 4) was a viral receptor. This confocal micrograph shows that PVRL4 is localized to adherens junctions as well as the apical surfaces of susceptible MCF7 breast cancer cells. PVRL4 was labeled with anti-PVRL4 antibodies and detected with fluorescent mouse anti-goat antibodies. Nuclei were revealed with TO-PRO-3. Non-susceptible cells failed to express PVRL4. Measles virus may have oncolytic properties against tumors that over-express PVRL4 (see Noyce et al., doi:10.1371/journal.ppat.1002240).
Image Credit: Ryan S. Noyce and Christopher D. Richardson, Department of Microbiology & Immunology, Dalhousie University
Citation: (2011) PLoS Pathogens Issue Image | Vol. 7(8) August 2011. PLoS Pathog 7(8): ev07.i08. https://doi.org/10.1371/image.ppat.v07.i08
Published: August 25, 2011
Copyright: © 2011 Noyce, Richardson. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Measles virus infects primary human airway epithelial cells and cell lines derived from lung, breast, and colon adenocarcinomas. Microarray analysis of genes expressed in cells susceptible and non-susceptible to measles virus revealed that PVRL4 (Nectin 4) was a viral receptor. This confocal micrograph shows that PVRL4 is localized to adherens junctions as well as the apical surfaces of susceptible MCF7 breast cancer cells. PVRL4 was labeled with anti-PVRL4 antibodies and detected with fluorescent mouse anti-goat antibodies. Nuclei were revealed with TO-PRO-3. Non-susceptible cells failed to express PVRL4. Measles virus may have oncolytic properties against tumors that over-express PVRL4 (see Noyce et al., doi: 10.1371/journal.ppat.1002240).
Image Credit: Ryan S. Noyce and Christopher D. Richardson, Department of Microbiology & Immunology, Dalhousie University