Figures
Listeria monocytogenes invasion of the villus tip.
Three-dimensional confocal image reconstruction of a murine intestinal villus tip infected with L. monocytogenes expressing InlAm, InlB, and green fluorescent protein (GFP). Six hours after orogastric gavage, the terminal ileum was imaged with phalloidin in red to visualize actin, TO-PRO-3 in blue to see the nuclei, and GFP in green to see invading bacteria. Several replicating cytosolic bacteria surrounded by actin clouds or tails have invaded the villus tip epithelial cells. InlB accelerates invasion of the villus epithelium (see Pentecost et al., doi:10.1371/journal.ppat.1000900).
Image Credit: Mickey Pentecost and Manuel R. Amieva, Stanford University School of Medicine
Citation: (2010) PLoS Pathogens Issue Image | Vol. 6(5) May 2010. PLoS Pathog 6(5): ev06.i05. https://doi.org/10.1371/image.ppat.v06.i05
Published: May 27, 2010
Copyright: © 2010 Pentecost, Amieva. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Three-dimensional confocal image reconstruction of a murine intestinal villus tip infected with L. monocytogenes expressing InlAm, InlB, and green fluorescent protein (GFP). Six hours after orogastric gavage, the terminal ileum was imaged with phalloidin in red to visualize actin, TO-PRO-3 in blue to see the nuclei, and GFP in green to see invading bacteria. Several replicating cytosolic bacteria surrounded by actin clouds or tails have invaded the villus tip epithelial cells. InlB accelerates invasion of the villus epithelium (see Pentecost et al., doi:10.1371/journal.ppat.1000900).
Image Credit: Mickey Pentecost and Manuel R. Amieva, Stanford University School of Medicine