Fig 1.
(A) Coverage of the meta-transcriptomic sequencing reads mapped to the BSVV1 reference sequence (library KAZ-SL-20). Reads spanning both ORF1 and ORF2, as well as both ORF2 and ORF3, are highlighted. (B) Predicted structure of the full-length genome sequence of BSVV1 and the three predicted ORFs, as well as functional motifs. (C) The overlapping primers designed to amplify the BSVV1 genomic fragments using RT-PCR for Sanger sequencing, where F23/R23 is a primer that spans the three regions of ORF1, ORF2, and ORF3.
Fig 2.
Schematic genome structure of BSVV1 and related viruses.
Vmethyltransf: viral methyltransferase, FtsJ: ftsJ-like methyltransferase, RdRp: RNA-dependent RNA polymerase, Nuc: nucleocapsid protein.
Fig 3.
Predicted motifs of ORF2 of BSVV1 and representative members of the order Bunyavirales.
The representative members of the Bunyavirales used in this analysis included the viruses most closely related to BSVV1 and those from different families within the Bunyavirales (downloaded from GenBank). The motifs were predicted using the online service provided by MEME. Different colors represent different functional motifs.
Fig 4.
Phylogenetic analysis of the conserved palm subdomain of BSVV1 and representative members of the order Martellivirales.
The phylogeny was estimated using the palm subdomain of RdRp protein sequences of BSVV1 and representative viruses from the Martellivirales. The reference sequences were downloaded from GenBank and aligned using Mafft. Phylogenetic analysis was performed using IQ-TREE, with the best-fit LG+F+I+G4 amino acid substitution model and 1000 bootstrap replicates. The scale bar represents the number of amino acid substitutions per site, and only bootstrap values >80% are shown.
Fig 5.
Integrating analysis of mass spectrometry data of viral ORF1, ORF2, and ORF3 protein products of samples 1, 2, and 3 of the BSVV1 positive pool LC20181207.
(A) Primary sequence coverage map of ORF1, ORF2, and ORF3 protein products, with the identified peptides highlighted in red. (B, C) Annotated MS/MS spectrometry of representative peptides from the ORF1 protein products. (D, E) Annotated MS/MS spectrometry of representative peptides from the ORF2 protein products. (F, G) Annotated MS/MS spectrometry of peptides from the ORF3 protein products.