Fig 1.
Treatment of CyLCV-associated post-transplant lymphoproliferative disease in HSCT recipient MCMs is challenging.
(A) Drug regimens with timepoints aligned to graphs in B, C, with colors corresponding to each MCM shown in graphs. (B) Longitudinal plasma (top) and cell-associated (bottom) CyLCV DNA viral loads. Limit of quantification (LOQ) = 60 copies/mL for plasma, 260 copies/million cells for cell-associated. Undetectable and below LOQ measurements graphed at the LOQ. Colored vertical dashed lines represent timepoints of anti-CD20 monoclonal antibody administration. Graphs show mean viral loads of two qPCR replicates. (C) Longitudinal CD20+ B cell (top) and CD79a+ B cell (bottom) absolute counts in blood. (D, E) Representative flow cytometry plots showing intracellular Ki67 staining of B cells and T cells in MCM 36481 (D) and 36478 (E) tissues. Plots are gated on live, CD45+ singlets. Numbers indicate cell frequencies within each quadrant. Bolded tissues represent affected tissues positive for lymphoma. (F) Table summarizes tissue sections with signs of lymphoproliferative disease assessed by H&E and presence of cells double-positive for CD20 and EBNA2 assessed by dual immunohistochemistry. *section not specified. (G) Cell-associated CyLCV DNA viral loads of necropsy tissues. White patterned bars denote tissues positive for lymphoma. Bars show mean ±SD of two qPCR replicates. Limit of quantification (LOQ) = 260 copies/million cells for cell-associated. Undetectable and below LOQ measurements graphed at the LOQ. Colored crosses next to datapoints indicate time of euthanasia/death for each MCM. ART = antiretroviral therapy, RIC = reduced intensity conditioning, SmLN = submandibular lymph node, AxLN = axillary lymph node, IngLN = inguinal lymph node, MesLN = mesenteric lymph node, TOD = time of death.
Fig 2.
Generation and infusion of CyLCV-BLCLs into immunosuppressed LCV model MCMs.
(A) Cell-associated (left) and supernatant (right) CyLCV DNA viral loads in ex vivo-generated CyLCV-BLCL cultures using CyLCV from PTLD-experiencing HSCT recipient MCM. Bars show mean ±SD of two qPCR replicates. Hashtags (#) indicate CyLCV-BLCL lines not generated. (B) Study outline for LCV model MCMs. (C) Table summarizing dosing of autologous CyLCV-BLCL infusions into LCV model MCMs. (D) Longitudinal plasma (top) and blood cell-associated (bottom) CyLCV DNA viral loads. LOQ = 60 copies/mL for plasma, 260 copies/million cells for blood cells. Graphs show mean viral loads of two qPCR replicates. Drug regimens and CyLCV-BLCL infusion timepoints indicated above graphs with colors corresponding to each MCM shown in graphs. Colored crosses next to datapoints indicate time of euthanasia/death for each MCM.
Fig 3.
Majority of CyLCV-BLCL-infused, immunosuppressed LCV model MCMs develop CyLCV-associated lymphoproliferative disease.
(A) Table summarizes tissue sections with signs of lymphoproliferative disease assessed by H&E staining and presence of cells double-positive for CD20 and EBNA2 assessed by dual immunohistochemistry. (B) Representative flow cytometry plots showing intracellular Ki67 staining of B cells and T cells in MCM 34667 necropsy tissues. Plots are gated on live, CD45+ singlets. Numbers indicate cell frequencies within each quadrant. Bolded tissues represent affected tissues positive for lymphoma. (C, D) Summary graphs of flow cytometry staining of LCV model MCM necropsy tissues. Frequencies of CD20+ B cells and CD79a+ B cells among live CD45+ cells (C). Frequencies of Ki67+ cells among live CD20+ B cells and CD79a+ B cells (D). White patterned bars denote affected tissues positive for lymphoma. Bars not shown indicate samples not assayed. (E) Cell-associated CyLCV DNA viral loads of necropsy tissues. White patterned bars denote affected tissues positive for lymphoma. Bars show mean ±SD of two qPCR replicates. LOQ = 260 copies/million cells. Asterisks (*) indicate samples that measured below LOQ. Other bars not shown indicate samples not assayed. PancrLN = pancreatic lymph node, AxLN = axillary lymph node, IngLN = inguinal lymph node, MesLN = mesenteric lymph node, IleocLN = ileocecal lymph node, HepLN = hepatic LN.
Fig 4.
18F-FDG PET of model MCM 38143.
(A-H) Longitudinal maximum standardized uptake values (SUVmax) for 18F-FDG in various tissues from scans of model MCM 38143. Gray boxes denote the period of daily tacrolimus (tac) treatment. Purple arrowheads denote timepoints of belatacept administration. Longitudinal 38143 plasma CyLCV DNA viral loads are overlayed on right adrenal gland graph (E, right axis), line shows mean viral load of two qPCR replicates. LOQ = 60 copies/mL plasma. Undetectable and below LOQ measurements graphed at the LOQ. L = left, R = right, LN = lymph node.