Fig 1.
Sequence preference of mRNA editing and stop codon editing in F. graminearum editing in fungi.
(A) A-to-I mRNA strongly favors U at the −1 position. (B) A-to-I editing of UAG leads to premature stop codon correction (PSC) and stop-loss editing. Editing of UAA to UGA retains the stop codon. PS, CS, and IS represent premature, canonical, and in-frame stop codons, respectively. (C) PSC, stop-loss, and stop-retaining editing events in F. graminearum.
Fig 2.
A model for editing of A34 in tRNA and A-to-I mRNA editing by FgTad2 and FgTad3.
In vegetative hyphae, the FgTad2L and FgTad3 heterodimer functions as regular ADAT to edit A34 in the anticodon loop of tRNA. During sexual reproduction, the expression of short isoforms and stage-specific cofactors, including Ame1 and Fip5, enable the editing of adenosines in the hair loops (favored) and stems (dsRNA regions) of mRNA by the FgTad2S-FgTad3-cofactor protein complex.