Fig 1.
Distinct substrates of SpoT/MESH1 and the conserved biological processes and phenotypic features of the bacterial (left) and mammalian (right) stringent response.
Fig 2.
Comparison between Rel/SpoT and MESH1.
(a) Crystal structure of Thermus thermophilus Rel in complex with ppGpp (PDB: 6S2T). The hydrolase domain of Rel/SpoT hydrolyzes ppGpp to yield GDP and pyrophosphate. (b) Crystal structure of MESH1 in complex with NADPH (PDB: 5VXA). MESH1 hydrolyzes NADPH to yield NADH and phosphate. Rel and MESH1 are shown in the cartoon model, ppGpp and NADPH in the stick model, and active site Mn2+ ions shown in the sphere model.