Table 1.
H7N9 viruses isolated between February 2018 and December 2019.
Fig 1.
Phylogenetic analyses and genotypes of H7N9 highly pathogenic avian influenza viruses.
The phylogenetic trees of the HA (A) and NA (B) genes were rooted to A/chicken/Rostock/45/1934 (H7N1) and A/chicken/Italy/22A/1998 (H5N9), respectively. The viruses sequenced in this study are shown in red in the phylogenetic trees. (C) Genotypes of H7N9 and H7N2 viruses and the hosts in which these genotypes were detected. The genotypes of the viruses isolated between February 2017 and January 2018 were reported previously [7]; the viruses isolated between February 2018 and December 2019 were analyzed in this study. The numbers of strains of each genotype are provided in parentheses.
Fig 2.
Replication and virulence of H7N9 viruses in mice.
(A) Viral titers in organs of mice after inoculation with 106 EID50 of different viruses. Three mice from each group were killed on Day 3 p.i., and virus titers were determined in eggs. Color bars show the mean, and the error bars represent standard deviations. The values labeled with a red star indicate that the virus was only detected in the organ of one mouse. The dashed lines indicate the lower limit of virus detection. (B) Changes in body weight in the groups of five mice after inoculation with 106 EID50 of different viruses. (C) Mouse-lethal doses of the CK/IM/SD010/19 virus.
Table 2.
Replication and virulence of H7N9 viruses in chickens and ducksa.
Fig 3.
Receptor-binding properties of H7N9 representative viruses isolated between 2013 and 2019.
The binding of H7N9 viruses to two different glycans (α-2,3-glycans, blue; α-2,6-glycans, pink) was assessed. The data shown are the means of three repeats, the error bars indicate standard deviations.
Fig 4.
Antigenic cartography of H7N9 viruses.
The antigenic map was generated by using the HI assay data shown in S2 Table. Each unit in the coordinate represents a 2-fold difference in HI titer. The pink cubes represent the antisera generated from the indicated viruses. The red balls indicate the viruses used for antisera generation, and the green balls show the test viruses.
Fig 5.
Protective efficacy of H5/H7-Re2 trivalent inactivated vaccine against challenge with different H7N9 viruses in chickens.
HI antibody titers (A-E), virus shedding titers (F-J), and survival patterns (K-O) of chickens challenged with the H7N9 highly pathogenic viruses CK/GX/SD098/17 (A, F, and K), CK/SX/SD006/18 (B, G, and L), PCK/LN/SD004/19 (C, H, and M), CK/IM/SD010/19 (D, I, and N), and CK/LN/SD25/19 (E, J, and O). The dashed lines shown in A-E show the cutoff value for seroconversion and those in F-J show the lower limit of virus detection. Virus titers shown in F-J are the means from the birds that survived. A value of 0.5 was assigned to virus shedding-negative birds for statistical purposes. The asterisks indicate that the bird(s) died before that day, and therefore virus shedding data were not available for statistical analysis. All of the chickens in these control groups died within 5 days of challenge. The letter “a” indicates p < 0.001 compared with the corresponding titers of the control birds.
Fig 6.
Key mutations in HA that contributed to the antigenic drift of the 2019 H7N9 viruses.
(A) Amino acid differences in the HA1 protein of the representative H7N9 viruses CK/GX/SD098/17 and CK/IM/SD010/19. The key amino acids in the head of the HA1 trimer that differ between the two viruses are shown in red. The colored boxes show different antigenic regions (site A to site E). The 2D structure of the HA1 protein of CK/GX/SD098/17 (B) and CK/IM/SD010/19 (C), and the 3D structure of the HA1 protein of CK/GX/SD098/17 (D) were obtained by using SWISS-MODEL; images were drawn with Pymol software. The numbers show the positions of the key amino acid in the head of the HA1 trimer that are different in the representative viruses. (E) HI titers of different H7N9 mutants against H7-Re2 antiserum and H7N9 monoclonal antibodies. (F) Mobility of H7N9 avian influenza HA1 protein analyzed by SDS-PAGE and Western blotting.