Fig 1.
EM image and the overall structure of GNNV-LP.
(A) A representative negative-staining EM image of the purified GNNV-LPs after self-assembly. (B) A ribbon presentation of the subunit C of GNNV-LP. The disordered N-ARM (residue 1−33, gray), N-arm (residues 34−51, magenta), the S-domain (residues 52−213, red), the linker region (residues 214−220, blue), the P-domain (residues 221−338, cyan) and Ca2+ ion (yellow sphere) are shown. (C) A topology diagram of GNNV CP with the helices and strands in cylinders and arrows, respectively. The 1D topology of the subunit C is color-coded as in B. (D) Surface domain-colored diagram (left) and central cavity (right) representations of the T = 3 GNNV-LP. The tip-to-tip distance is ~350 Å, the diameter of the central cavity is ~228 Å, and the spike protrusion on the capsid surface is ~47 Å. The S-domains of the subunits A, B and C are shown in orange, blue and red, respectively, and the P-domains are shown in cyan. The structure of the GNNV-LP is viewed along the I2, I3 and I5 axes.
Fig 2.
Structural organizations of the N-arm and the β-annulus.
(A) Two N-arms from the subunit-C/C dimer along the I2 interface. N-arms are colored in magenta. Two iASUs are shown in white and orange. (B) A representation of the hexameric capsomers and the β-annulus as viewed along the I3 axis from the inner surface. Three iASUs are shown in cyan, green and orange, respectively (left). Residues 36−41 of three different C-subunits form a β-annulus structure as an enlarged central section (right). The hydrogen bonds are shown with blue dotted lines. (C) A comparison of the N-arms with an alignment of the S-domains (subunit C) from four different representative viruses: SeMV (green), SMSV (yellow), RyMV (blue) and GNNV (cyan) with a nomenclature diagram. The orientations of the N-arm between GNNV and SeMV are towards opposite different I3 axes (black triangle).
Fig 3.
Trimeric interactions of the P-domain with Ca2+ ions and the functional role of PEG during GNNV infection.
(A) The ribbon model of trimeric P-domains. The structure of trimeric P-domains (magenta, green and cyan) is shown with two Ca2+ ions (yellow spheres) bound at the top region. (B) The electrostatic surface potential of the trimeric P-domains. A top view of the trimeric P-domains is rotated 90° along the horizontal axis from A, and colored in red and blue for negative and positive charges viewed along the three-fold axis. (C) The calcium-binding region of the trimeric P-domains. Two Ca2+ ions (yellow spheres) and two water molecules (blue) are coordinated with three sets of 273DxD275 motifs (sticks) from neighboring subunits colored in magenta, green and cyan, respectively. The hydrogen bonds are shown with orange dotted lines. (D) Sequence-alignment variables mapped onto the surface of the P-domain from different genotypes of betanodavirus. The hypervariable regions (yellow) from OSGNNV, DGNNV, RGNNV, BFNNV, SJNNV and TPNNV are represented on the surface of the trimeric P-domains (upper). The comparison of representative T4 genomic regions of the P-domains of different genotypes of betanodavirus is shown (lower). Strictly variants and similar residues are colored in yellow (as in D, upper) and green, respectively. (E) Improvement of GNNV infection by PEG treatment. PEG8000 assists GNNV infection in GF-1 cells. Intracellular GNNV RNA2 copies in GF-1 cells were determined by real-time qPCR in log scales after GNNV infection at the indicated time. Data are represented as mean ± SD of three independent experiments and analyzed by one-way ANOVA test, *P < 0.05; **P < 0.01; ***P < 0.001.
Table 1.
Data collection, phase extension and refinement statistics.
Fig 4.
(A) Surface presentations of the monomers engaged in pentameric interactions in the T = 3 and T = 1 GNNV capsids. Each iASU is viewed along the I5 axis and shown in green, wheat, cyan, yellow and magenta colors, respectively. The diameters of three capsids are indicated. (B) A superimposition of the calcium-binding regions of the S-domain between the T = 3 GNNV-LP and the T = 1 delta-P-domain mutant. Gln100, Asp130, Asp133, Ser170 and Glu213, which participate in the Ca2+ coordination in the T = 3 GNNV-LP (yellow), exhibit conformational changes in the T = 1 delta-P-domain mutant (green). The hydrogen bonds are indicated with blue dotted lines. (C) A comparison of the spatial relationship of subunit pairs in the T = 3 GNNV-LP and the T = 1 delta-P-domain mutant. Subunit packing and the nomenclature of the T = 3 and the T = 1 capsids are shown. Rotation angles (°) and translation distances (Å) are identified and compared between different subunit pairs of the T = 3 and the T = 1 GNNV capsids.
Fig 5.
Evolutionary lineages and self-assembly mechanisms of GNNV.
(A) Structural characteristics of different categories of RNA viruses. Simplified topology cartoons (β-strand, rectangles; jelly-roll structure, capsules; β-barrel; ovals) of CPs from different viruses, including FHV, IBDV, GNNV, Orsay virus, HEV and tombusvirus are shown. S-domains are colored in blue; P-domains are colored in smudge, gray and orange, respectively; the P1- and P2-domains are indicated in magenta and pink, respectively. The Ca2+ ion in the P-domain of GNNV is labeled as a green sphere. The direction (from N- to C-terminal) of the linker region of each CP is indicated with arrows. (B) Diagram showing the putative self-assembly process of the T = 3 and the T = 1 GNNV capsids. The P-domain and Ca2+ ions (yellow spheres) encode information that controls trimerization of the GNNV CPs for the putative pentameric and hexameric trimers. The N-ARM guides the assembly of the complete T = 3 capsid.