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Fig 1.

Maximum likelihood phylogeny inferred using RAxML v. 8.0.0 employing GTRCAT model and 1,000 bootstrap replicates.

Bootstrap branch support above 80% is shown. Multiple sequences in the same species are collapsed. Genera included in the family Ajellomycetaceae are shown, with the exception of Lacazia loboi, which could not meaningfully be aligned.

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Fig 2.

Clinical, pathological, and mycological facets of a novel Emmonsia-like fungus reported from South Africa.

Top left: Hyperkeratotic skin lesions in a patient with disseminated Emmonsia disease (published with patient consent; courtesy of Dr. Tabie Greyling, Stellenbosch University). Top right: Peripheral blood smear showing neutrophils with multiple phagocytosed yeast-like cells (Wright-Giemsa staining x1,000). Bottom right: Electron microscopy image of conidia (courtesy of Dr. Monica Birkhead, National Institute of Communicable Diseases). Bottom left: Light microscopy image of conidiophores and conidia (x1,000).

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Fig 3.

Timeline of human cases of disease caused by classical Emmonsia species (with adiaspores at 37°C) and novel Emmonsia-like species (with yeast cells at 37°C).

Vertical bars represent number of cases during 5-year intervals, as determined by literature review. The increase in cases of diseases caused by novel Emmonsia-like species occurring between 2006 and 2015 is primarily driven by recognition of HIV-associated cases in South Africa following the introduction of molecular identification protocols for dimorphic fungal infections in 2008.

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