Table 1.
Survival times of Bv109I and Bv109M after primary transmission of CWD.
Figure 1.
Histopathological and immunohistochemical analysis of Bv109I inoculated with Bv109ICWD.
(A) Serial sections showing neocortex (NC), hippocampus (Hp) and thalamus (Th), stained by haematoxylin and eosin and (B) by immunohistochemistry for PrPSc with SAF84 mAb - bar 500 µm. (C) and (D): magnification of the ventral thalamic nucleus (star) from (A) and (B), respectively - bar 50 µm.
Figure 2.
Lesion profiles of Bv109I infected with CWD following primary transmission and third passage.
Pathological phenotypes observed after primary transmission and third passage are shown in (A) and (B), respectively. CWD1, 2 and 4 from elk, are shown in red, green and violet lines with open circles, respectively; CWD3 from mule deer, in blue line and closed circles; CWD5, 6 and 7 from W-T deer in red, green and violet dashed lines and closed triangles, respectively. Vole-adapted sheep scrapie is shown in black dashed line and closed circles. Brain-scoring areas are: medulla (1), cerebellum (2), superior colliculus (3), hypothalamus (4), thalamus (5), hippocampus (6), septum (7), retrosplenial and adjacent motor cortex (8), cingulate and adjacent motor cortex (9).
Figure 3.
Western blot analysis of the PrPres level in the brain of CWD-affected voles.
(A) Immunoblot of proteinase K–resistant PrPSc (PrPres) from one original elk isolate (CWD1) and from voles infected with CWD1, 3 and 5. (B) Comparison of PrPres amount and glycoprofile between Bv109I-adapted CWD and Bv109M-adapted sporadic CJD, sheep scrapie and cattle BSE, at third passage. Membranes were probed with SAF84. Molecular size markers are shown in kilodaltons on the right.
Table 2.
Survival times of Bv109I and Bv109M after subsequent passages of CWD.
Figure 4.
Regional distribution, by PET-blot, of PrPres following the third passage of CWD1, CWD3, CWD5 and sheep scrapie in Bv109I.
Coronal sections of the forebrain representing: telencephalon (A), diencephalon (B), midbrain (C) and hindbrain (D). In the lower part of the figure, the labelled coronal sections of negative control brain from 150 days old Bv109I are shown: VP, ventral pallidum; NC, neocortex; Hp, hippocampus; Th, thalamus; GN, geniculate nuclei; SN, substantia nigra; ICN, interposed cerebellar nucleus; MO, medulla oblongata.
Figure 5.
End-point titration of Bv109ICWD infectivity in Bv109I.
Triangles represent individual survival times. Voles that did not show signs of infection after 450 d.p.i. are plotted in compressed form after the x axes break point. The mean survival times (days ± standard deviation) and the numbers of diseased/inoculated voles are indicated on the right of each chart.
Figure 6.
Biological properties of in vitro-generated Bv109ICWD (Bv109ICWDPMCA).
A) Lesion profiles of Bv109I infected with Bv109ICWDPMCA following primary transmission (open circles) and second passage (closed diamonds), compared with Bv109I infected with Bv109ICWD (triangles). Brain-scoring positions are described in Figure 2. B) Regional distribution, by PET-blot with SAF84 mAb, of PrPres in Bv109I infected with Bv109ICWDPMCA following primary transmission and second passage compared with the control group (Bv109ICWD). Brain areas are as in Figure 4.