Figure 1.
Low dose intragastric infection with mouse-adapted L. monocytogenes InlAm.
Female mice were infected with the indicated inocula by oral gavage and the total number of L. monocytogenes CFU (luminal plus cell-associated) in (A) the small intestine, (B) mesenteric lymph nodes (LN), (D) liver, and (E) spleen was determined 24 hpi. Symbols represent values for individual mice; horizontal lines indicate the mean for each group. Pooled data from 2–3 separate experiments are shown. (C) Mean values +/− SE for L. monocytogenes found in either the luminal contents or cell-associated (adherent plus intracellular) in tissue homogenates 24 h after infection of B6 mice (n = 4) are shown. Dashed horizontal lines indicate the limit of detection for each tissue.
Figure 2.
BALB mice are more susceptible than B6 mice to L. monocytogenes infection acquired by ingestion of contaminated food.
Female BALB (white squares) or B6 (black squares) mice were fed 3–5×108 CFU of Lm InlAm either at noon or 9 PM (night) and the number of L. monocytogenes present in each tissue was determined over time. In panels A, B, E, F, and G, groups of mice were sacrificed 1, 3, 5, and 7 dpi. In panels D, H, and I the indicated organs were harvested 5 dpi. For spleen, liver, small intestines, colon, gall bladder and brain, mean values +/− SD for data pooled from 2 separate experiments (n = 8 mice per group) are shown. Sample groups for fecal analysis in panels (C) and (G) included 8 mice at all time points for night infection and 22 mice (1 dpi), 15 mice (2 dpi), 14 mice (3 dpi), 12 mice (4 dpi), 9 mice (5 dpi), or 4 mice (7 & 8 dpi) for groups infected at noon. Values for BALB mice that were significantly different from the corresponding B6 group by Mann-Whitney analysis are marked with asterisks. Dashed lines indicate the limit of detection.
Figure 3.
Female BALB, but not B6 mice, are more susceptible than males to food borne listeriosis.
Groups of mice (n = 4) were fed 5×109 Lm InlAm at night and bacterial loads were determined 4.5 dpi. Mean values +/− SD from one of two separate experiments are shown. Dashed lines indicate limits of detection.
Figure 4.
InlA enhances, but is not required for colonization of the murine intestines.
Female BALB mice were co-infected at night with a 1∶1 mixture of Lm InlAm and either wild type (wt) Lm EGDe or inlA deletion mutant (ΔinlA) for a total inoculum of 7–9×108 CFU. The total number of each Listeria strain found in either the ileum or colon was determined at both 16 and 60 hpi. Only the terminal third of the small intestine (approximating the ileum) was harvested because a pilot study showed that the majority of L. monocytogenes colonization occurred in the distal portion of the small intestine (Fig. S3). Pooled data from three separate experiments are plotted as competitive indices (CI) to show the ratio of either wt/InlAm or ΔinlA/InlAm recovered from each individual mouse. The geometric mean for each group was compared to the theoretical value of 1.0 and the fold difference is shown in parentheses above.
Figure 5.
InlAm enhances spread to the mesenteric lymph nodes and spleen.
Female BALB mice were co-infected at night with a 1∶1 mixture of Lm InlAm and either wild type (wt) Lm EGDe or an inlA deletion mutant (ΔinlA) for a total inoculum of 5–7×108 CFU and the total number of each Listeria strain found in the mesenteric lymph nodes (MLN), spleen, liver, and gall bladder was determined. Pooled data from at least two separate experiments are shown. In panel A, the data are plotted as competitive indices (CI) to show the ratio of either wt/InlAm or ΔinlA/InlAm recovered from each individual mouse at 60 hpi. The geometric mean for each group was compared to the theoretical value of 1.0 and the fold difference is shown in parentheses above. The actual number of CFU recovered in each mouse after wt (triangles) plus InlAm (squares) co-infection or ΔinlA (circles) plus InlAm are shown in panels B & C, respectively. Horizontal bars indicate mean values for each group; statistical significance was assessed by student's t test. The limit of detection in each tissue is marked by a dashed line.
Figure 6.
L. monocytogenes reside in both extracellular and intracellular compartments in the gut mucosa.
(A) Intestinal tissues were separated into three fractions: the mucus layer (muc), epithelial cells (EC) and the underlying lamina propria (LP) cells. BALB mice (n = 4 per time point) were fed 2×109 Lm InlAm at night. (B) Mean values +/− SE for the total number of Lm InlAm in the mucus layer and (C) the number of intracellular (gentamicin resistant) and extracellular (supernatant fraction) Lm InlAm in the LP and EC layers of the ileum and colon are shown. Data from one of two separate experiments are shown.
Figure 7.
InlAm promotes persistence of L. monocytogenes in the lamina propria of the colon.
Female BALB mice were co-infected at night with a 1∶1 ratio of InlAm and wild type (wt) Lm EGDe for a total inoculum of 7–9×108 CFU. At 36 and 60 hpi, the ileum and colon from each mouse was fractionated, and the total number of each strain found in the mucus (A, B) and the number of intracellular (GentR) Listeria in either epithelial cells (A, C) or lamina propria cells (A, D) was determined. Pooled data (n = 8) from two separate experiments are shown. In panel A, the data are plotted as competitive indices (CI) to show the ratio of either wt/InlAm recovered from each mouse 60 hpi. The geometric mean for each group was compared to the theoretical value of 1.0 and the fold difference is shown in parentheses above. The actual number of InlAm CFU (squares) or wt CFU (triangles) recovered from each fraction are shown in panels B, C, and D. Horizontal bars indicate mean values for each group; statistical significance was assessed by student's t test. The limit of detection in each tissue is marked by a dashed line.