Figure 1.
Clinical appearance and histology of the trichodysplasia spinulosa patient.
Facial appearance at presentation is shown in panel A. Note the thickened skin, particularly on the nose and in the eyebrow region accompanied by central alopecia. Apart from the eyebrows and nose, papules are seen on the cheeks, chin, forehead and ears. Especially on the nose, but occasionally also in cheeks and chin, keratotic spicules protruded from the enlarged follicular orifices. Panel B shows a close-up of the nose at presentation with numerous papules and spicules. In panel C is shown a section of a formalin-fixed, paraffin-embedded biopsy of a hyperkeratotic follicular papule from the forehead. The epidermis reveals enlarged, hyperplastic hair bulbs and hypercornification within a distended follicular infundibulum (HE stain, 10×). Panel D shows a detail of the nose 3 months after topical cidofovir treatment. Papules and spicules have largely resolved and hairs have regained growth.
Figure 2.
Restriction analysis of the RCA product.
EcoRV and XbaI digestion revealed one band of around 5000 bp. EcoRI digestion produced two bands of around 3600 and 1600 bp. After HindIII digestion 3 bands were visible of around 3000, 1500 and 400 bp. Sequence analysis later showed that HindIII digestion in fact produced four fragments of which the smallest, 378 and 364 bp, coincided on gel.
Figure 3.
Indicated are the five identified ORFs representing the putative “early” genes encoding small and large T antigen, and the putative “late” genes encoding VP1, VP2 and VP3. The NCCR is placed on top and contains the putative ori. Nucleotide position 1 was chosen within the NCCR in the large T binding region. For a detailed view of the NCCR, see Figure 4A.
Figure 4.
Detail of the TSV non-coding control region.
Indicated are the putative large T-bindings sites located on both strands (gray-shaded boxes), the putative ori and putative TATA box (A/T-rich elements). Downstream of this area another two TSV putative large T-bindings sites are located, as well as one from KIV [1]. Nucleotide positions 1 are shown in white and underlined, except for MCV where the position 1 is shown in bold and underlined. For MCV two isolates are shown, MCV 339 and MCV350 [3].
Table 1.
Overview of putative TSV genes and proteins.
Table 2.
Amino acid sequence similarities between putative TSV proteins and those of other polyomaviruses.
Figure 5.
Amino acid sequence of the TSV large T protein.
Indicated are three major domains found in the large T amino acid sequence; the J-domain (blue-shaded box), the ori DNA-binding domain (yellow-shaded box) and the helicase domain (green-shaded box). Within the J-domain the putative locations of conserved region 1 and the HPDKGG motif are depicted. Downstream of the J-domain, a pRb family-binding motif and nuclear localization signal are located. In the helicase domain, a zinc finger motif, NTPase-binding ‘Walker’ motifs A and B and an helicase SF3 (superfamily 3) motif C are located.
Figure 6.
Phylogenetic analysis of known polyomaviruses and TSV.
Bayesian posterior probability trees are shown for VP1 (A), VP2 (B), large T antigen (C) and a concatenation of all three (D). Numbers at branch points represent posterior probability support values and the scale bar is given in average number of substitutions per amino acid position. Major clades in the trees are highlighted using colored triangles. The following viruses are shown: Simian virus 40 (SV40), Goose hemorrhagic polyomavirus (GHPyV), Simian virus 12 (SV12), Squirrel monkey polyomavirus (SquiPyV), Finch polyomavirus (FPyV), Crow polyomavirus (CPyV), Bovine polyomavirus (BPyV), Merkel cell polyomavirus (MCPyV), WU Polyomavirus (WUPyV), KI polyomavirus Stockholm 60 (KIPyV), Budgerigar fledgling polyomavirus (BFPyV), African green monkey polyomavirus (AGMPyV), JC polyomavirus (JCPyV), BK polyomavirus (BKPyV), Murine polyomavirus (MPyV), Murine pneumotropic virus (MuPtV), Myotis polyomavirus VM-2008 (MyPyV), Bornean orangutan polyomavirus isolate Bo (OraPyV1), Sumatran orangutan polyomavirus isolate Pi (OraPyV2) and Trichodysplasia spinulosa-associated polyomavirus (TSPyV).