T cell responses to F-MLV are partially dependent on Myd88.
Myd88 knockout mice and heterozygous littermates were infected with F-MLV for 14 days. (A) Splenocytes were stained with TCRβ, CD8α, and the DbGagL tetramer, and the proportion of TCRβ+ CD8+ cells that were GagL+ was measured. A representative FACS plot gated on TCRβ+ CD8+ cells is shown in the upper panel. For the bar graph in the lower panel, each bar is the average of four mice. (B) Splenocytes were stained for TCRβ, CD4, and intracellular interferon-γ. The proportion of TCRβ+ CD4+ T cells that expressed interferon-γ was measured. Each bar is the average of four mice. The data shown are representative of three independent experiments.
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