Figure 1.
M. incognita proteins were collected from the aqueous medium (the secretome) and from extracts of worms. Proteins in the tomato root exudates (TRE) that diffused across a 3,500Da filter membrane were also collected. Proteins were identified by nanoLC ESI MS/MS. A, B, C and D are Protein databases used for protein identification, complementary information for each database are available on Table S3.
Figure 2.
Distribution of secreted proteins identified in the protein databases.
Venn diagram showing the distribution of secreted proteins identified using the M. incognita, Other Nematode, or Plant protein databases. A complete description of all databases is shown in Table S3.
Figure 3.
Relative abundance of secreted proteins compared to extracts from intact nematodes.
Spectrum counting was used for relative protein quantification compating the secretome to the intact nematode proteome. The number of valid MS/MS spectra from each protein was normalized to the total MS/MS spectra number of each dataset. The normalized spectrum count ratio of each protein (secretome/whole worm proteome) was used to evaluate if the protein was enriched in the secretome. Circle represents all secreted proteins identified in this study; Triangle represents proteins previously reported to be in the secretome (A complete description of these proteins is shown in Table S5); Diamond represents proteins that we found in M. incognita secretome and that could play a crucial role in the establishment of the host-pathogen compatibility. These proteins are discussed in detail.
Table 1.
Selected proteins from the M. incognita secretome.
Figure 4.
Localization of gene expression by in situ hybridization.
Digoxigenin-labeled antisense cDNA probes of selected gene clones were hybridized to transcripts expressed within cells of J2 stage Meloidogyne incognita. Sections of the nematode were incubated with antisense probes designed based on DNA sequence of the following contigs: A, CL1191Contig1_1; B, CL312Contig1_1; C, CL5Contig2_1; D AF100549 (β-1,4-endoglucanase), E, CL2552Contig1_1; F, CL321Contig1_1; G, AF402771 (calreticulin); H, CL480Contig2_1. M = metacorpus, SvG = subventral glands.