Figure 1.
Phylogenetic relationships of the PB2 gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using a maximal likelihood (ML) method.
All branch lengths are drawn to a scale of nucleotide substitutions per site. The tree is rooted using the oldest sequence, A/Brevig Mission/1/1918, and temporally ordered with the oldest isolates falling at the bottom left of the phylogeny and the newest isolates at the top right. Main phylogenetic sections that are separated by long trunk branches are numbered I–IX and colored as follows: section I is red, section II is orange, section III is yellow, section IV is light green, section V is dark green, section VI is light blue, section VII is absent due to the position of clade G within section VI (would otherwise be blue), section VIII is purple, and section IX is gray. The trunk branches separating these sections are labeled #1–#8 and highlighted in dark blue, with the bootstrap value supporting this branch highlighted in yellow and the number of amino acid changes that occur along each branch appearing above in red font. Ten main clades are labeled A–J, with supporting bootstrap values in light blue, and are colored as follows: clade A is dark red, clade B is orange, clade C is yellow, clade D is olive green, clade E is light blue, clade F is turquoise, clade G is fuchsia, clade H is gray, clade I is pink, and clade J is white. Clades that have undergone reassortment are outlined in red (in this case Clade D).
Figure 2.
Phylogenetic relationships of the PB1 gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Figure 3.
Phylogenetic relationships of the PA gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Figure 4.
Phylogenetic relationships of the HA gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Figure 5.
Phylogenetic relationships of the NP gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Figure 6.
Phylogenetic relationships of the NA gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Figure 7.
Phylogenetic relationships of the M1 gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Figure 8.
Phylogenetic relationships of the NS1 gene of A/H1N1 influenza viruses (n = 71) sampled globally from 1918–2006, estimated using ML.
Rooting, scale, and color scheme are the same as those used in Figure 1.
Table 1.
Number of amino acid changes occurring along the main trunk branches of phylogenetic trees of each genome segment of A/H1N1 virus.
Figure 9.
Schematic representation of the phylogenetic patterns of all eight A/H1N1 influenza virus genomes used in this study (Figures 1–8).
The main trunk (back-bone) lineage is taken from the HA phylogeny, with the nine sections I–IX and long trunk braches #1–8 corresponding to their respective positions on HA phylogeny and similarly color-coded. Clades A–J are also color-coded as in Figures 1–8. Each clade is positioned within a given phylogenetic section for all viral genome segments contained within the box. Viral genome segments are color-coded to show similarities in the phylogenetic patterns between multiple segments: PB2 and HA follow similar phylogenetic patterns and are colored in yellow boxes; PB1, NA, and M are phylogenetically similar and are shaded in orange boxes; PA and NP are in green boxes; NS follows a pattern unique to itself and is alone colored in a dark purple box.