Figure 1.
Gastrointestinal Colonization Levels of C3H/HeN Mice Fed C. albicans Strains SC5314 and CAF2–1
Mice were maintained on sterile water with 2 mg streptomycin/ml and 1,500 U penicillin G/ml throughout the duration of the experiment, and 0.2 mg gentamicin/ml was added once colonization was confirmed. Points represent results from individual animals, and horizontal lines represent the medians. n = 16 for each strain.
Table 1.
Immuosuppressive Regimens
Table 2.
Survival of Mice after Administration of Immunosuppressive Regimens
Table 2.
Extended.
Figure 2.
DSS-Induced Murine Gastrointestinal Mucosal Damage
Histological sections of cecums from mice colonized with C. albicans strain SC5314 with continuation of penicillin, streptomycin, and gentamicin in the drinking water then given water with 2.5% DSS for various periods with or without induction of neutropenia.
(A) No DSS, 200 μg of RB6-8C5 IP once.
(B) DSS for 3 d.
(C) DSS for 7 d.
(D) DSS for 7 d and after the third day of DSS mice were given 200 μg of RB6-8C5 IP once and observed for 96 h after the RB6-8C5 dose. Magnification: Objective 10×.
Sections in (A) and (B) have normal histologic appearance, whereas in panels (C) and (D) disruption of the epithelial barrier integrity was clearly seen.
Figure 3.
Viable Counts of C. albicans Strains SC5314 or CAF2–1 in the Livers of Deceased C3H/HeN Mice that Were Previously Colonized in the GI Tract with C. albicans and Subsequently Given Various Forms of Immunosuppression
Cy, cyclophosphamide; MTX, methotrexate; RB6, RB6-8C5 mAb; DSS, dextran sulfate sodium. Points represent results from individual animals. Horizontal lines with bars represent median with interquartile range when n > 3, otherwise only the median is shown.
Figure 4.
Gastrointestinal Colonization Levels of Female 6- to 8-Wk-Old C3H/HeN Mice Fed C. albicans Strains CAF2–1, HLC-54 (Δefg1/cph1), and BCa-210 (Δtup1)
Points represent results from individual animals, and horizontal lines represent the medians. n = 8 for CAF2–1, n = 7 for Δefg1/cph1 and Δtup1. Colonization levels with strain Δtup1 were significantly lower (p = 0.0003 by Mann Whitney test) compared with other two strains.
Figure 5.
Survival Curve of C3H/HeN Mice Fed C. albicans Strains CAF2–1, HLC-54 (Δefg1/cph1), or BCa-210 (Δtup1) and Subsequently Given mAb RB6-8C5 (200 μg IP) and Methotrexate (150 mg/kg/dose IP)
Median survival of mice colonized with strain Δefg1/cph1 was significantly higher than that of mice colonized with wild-type C. albicans CAF2–1 (p = 0.01, log rank test). Each group contained 8 mice.
Table 3.
Fungal Strains Used in This Study
Figure 6.
Schema for Murine Model of C. albicans Gastrointestinal Colonization and Dissemination after Administration of Immunosuppression
Table 4.
Translocation Study