Table 1.
dsRNA Treatments Tested for Their Effect on L. pneumophila Replication
Figure 1.
RNAi Targeting Multiple Secretory Components Decreases L. pneumophila Replication
dsRNA-treated Kc167 cells were incubated for 1 h with L. pneumophila at MOI = 1, washed, and incubated for 30 h prior to microscopic examination of replication vacuoles. The noted dsRNAs were added to Drosophila cells, and allowed to incubate with the cells prior to introduction of L. pneumophila, as described (see Materials and Methods). Untreated: no dsRNA added. The mean ± standard error is plotted.
Figure 2.
RNAi Targeting Cdc48/p97 and Cofactors Decreases L. pneumophila Replication in Drosophila and Human Tissue-Culture Cells
(A) dsRNA treated Kc167 cells were incubated for 1 h with L. pneumophila at MOI = 1, washed, and incubated for 30 h prior to microscopic examination of replication vacuoles; mean ± standard error.
(B) Drosophila Kc167 treated with dsRNA directed against Cdc48/p97, p47 or no dsRNA [30]. Cdc48/p97-treated cells (1 × 106) were lysed in SDS-PAGE buffer, separated by SDS-PAGE electorphoresis, and subjected to Western blotting with anti-Cdc48/p97 [35] and antitubulin (Serotec) as a loading control. RNA from p47-treated cells was collected using trizol reagent (Invitrogen), cDNA was prepared using Superscript reverse transcriptase (Invitrogen) and 25 cycles of PCR were completed against either actin or p47.
(C) HEK 293 cells were silenced for p47 or Cdc48/p97 [39], incubated with L. pneumophila at MOI = 2, and washed and incubated for 11 h prior to microscopic examination of replication vacuoles.
(D) HEK 293 cells were silenced for Cdc48/p97 or p47 [39] and subjected to Western blotting with anti-p97 or anti-p47. Antitubulin (Serotec) served as a loading control.
(E) Network of genes that interact with Cdc48/p97. Lines are color coded and weighted according to predicted confidence score for each interaction [36].
(F) RNAi of Cdc48/p97 cofactors as in (A), except only the mature (11+ bacteria) vacuoles were plotted.
Figure 3.
Cdc48/p97 Localizes to the L. pneumophila Vacuole
(A) Mouse BMDMs were incubated with L. pneumophila for 1 h at MOI = 1, fixed, and immunostained for human Cdc48/p97 and L. pneumophila. Inset is an enlargement of bacterium in panel. Cdc48/p97 associated with 63% ± 11% vacuoles harboring wild-type bacteria and 1.3% ± 1% dotA bacteria. (B) Kc167 cells were incubated with L. pneumophila for 1 h at MOI = 1; replication vacuoles were isolated, fixed, and immunostained with antibodies against Drosophila Cdc48/p97 (Ter94) [35] and L. pneumophila. Cdc48/p97 associated with 77% ± 6.9% vacuoles harboring wild-type bacteria and 1.3% ± 0.3% dotA bacteria.
Figure 4.
The Proteasome Promotes L. pneumophila Replication and Translocation of Bacterial Effectors causes Ubiquitination of the LCV
(A) Mouse BMDMs were pretreated for 1 h with proteasome inhibitor MG-132 or DMSO, incubated with L. pneumophila for 1 h at MOI = 1, washed, and incubated for 14 h. Infectious centers assay was performed as described.
(B) Mouse BMDMs were incubated with L. pneumophila for 1 h at MOI = 1, fixed at the indicated time, and immunostained with antipolyubiquitin and anti–L. pneumophila.
Figure 5.
Cdc48/p97 Removes Translocated Effectors from the Replication Vacuole
(A) Cdc48/p97 mediates the removal of LidA (green) from the LCV (red) during incubation with CM.
(B) Mouse BMDMs were incubated with L. pneumophila at MOI = 1 for 1 h in CM, washed, and fixed, or further incubated in CM as indicated. Cells were immunostained with anti-LidA and anti–L. pneumophila.
(C) Quantification of LidA-positive vacuoles from (B), showing mean of three replicates ± standard error; at least 100 cells counted per replicate.
(D) HEK 293 cells were silenced for Cdc48/p97 or p47 and incubated with wild-type L. pneumophila at MOI = 5 for 2 h in CM, washed, and fixed, or further incubated in CM for 16 h. Intact cells were immunostained for LidA and L. pneumophila.
(E) Quantification of LidA-positive vacuoles from (D). Untreated (black), si p47 (white), si Cdc48/p97 (gray). Mean of three replicates ± standard error; at least 100 cells counted per replicate.
Figure 6.
Cdc48/p97 Removes Polyubiquitinated Proteins from the Replication Vacuole
(A) HEK 293 cells were silenced for Cdc48/p97 or p47 and incubated with wild-type L. pneumophila at MOI = 5 for 2 hours in CM, washed, and fixed, or further incubated in CM for 16 h. Intact cells were immunostained for polyubiquitinated proteins and L. pneumophila.
(B) Quantification of polyubiquitin positive vacuoles from (A). Untreated (black), si p47 (white), si Cdc48/p97 (gray). Mean of three replicates ± standard error; at least 100 cells counted per replicate.
Figure 7.
Model for Functional Redundancy
The LCV captures secretory vesicles from multiple pathways. Sec22, TRAPP, and Arf1 promote vesicle movement through the IC, and the LCV captures membrane moving from the ER through the IC (white arrows). Alternatively, Sec22 and TRAPP may dock and tether vesicles on the LCV (filled arrows). In either case, Legionella replication is decreased when both pathways are disrupted.