Live attenuated vaccination protects aged chimeric ACE2 mice from severe SARS-CoV-2 pathogenicity in vivo
Fig 4
Lung infiltration and expression of interferon stimulated genes upon wt virus and LAVNsp16 infection in chimeric ACE2 mice.
(A-E) Lung infiltration by monocytes (CD45+CD11b+CD11c-), neutrophils (CD45+Gr-1high), natural killer cells (CD45+CD49b+), macrophages (CD45+CD11b+CD11c+), and CD8 + T cells (CD45+CD8+) was determined by antibody staining of cells present in bronchoalveolar lavage (BAL) and subsequent flow cytometry. Bars represent the mean + /- s.d. Statistical analysis were preformed using one-way ANOVA followed by Tukey’s test (A, B, D) or Kruskal-Wallis tests followed by Dunn‘s correction (C). (F) Transcript levels of the indicated interferon-stimulated genes in lung tissue of infected animals were determined by RT-qPCR and normalized to GAPDH (ΔΔCt). Relative mRNA levels compared to uninfected controls are plotted. Statistical analysis was performed using unpaired two-tailed t-tests. Samples originate from infected animals analyzed in Fig 3 at two, four, or seven days postinfection.