Prion shedding is reduced by chronic wasting disease vaccination
Fig 5
Representative Western blots showing PrPSc levels in third round of PMCA reactions seeded with urine of Ddi, Mmo vaccinated or CPG control KI mice in duplicates at different time points.
(A), 150 and 250 dpi and (B), 450 dpi samples were subjected to IOME followed by three rounds of PMCA. Negative control for the PMCA is naïve urine and positive control for the PMCA was naïve urine spiked (1:100) with brain homogenate of mouse-adapted CWD subjected to PMCA reaction. PMCA products were digested for 1 hour with 37 µg/ml PK at 37°C and were probed with anti-PrP mAb 4H11 (1:500). NBH: normal brain homogenate, used as negative PMCA control. (C-E), summary of the RT-QuIC analysis of prion seeding in the pooled urine of KI mice at (C) 150 dpi, (D) 250 dpi, and (E) 450 dpi. The heat map indicates the percentage of positive RT-QuIC replicates out of the total of four replicates analyzed. The scale ranges from 0 (all replicates were negative) to 100 (all replicates were positive). Graphs were generated using GraphPad Prism (version 10).