Prion shedding is reduced by chronic wasting disease vaccination
Fig 2
Humoral immune response of Ddi and Mmo vaccinated KI mice to immunogens.
ELISA plates were coated with Ddi immunogen (A, B) or Mmo immunogen (C, D). Sera were isolated after the 4th dose of vaccination (n = 8 per vaccination group.) All sera were diluted at 1:100, CpG mice sera were used as a negative control and GAM was used as secondary antibody at 1:4,000. One-way ANOVA was done followed by Tukey’s test. Statistical significance = **** p-value <0.0001, *** p-value = 0.0007. (E) End-point ELISA antibody dilutions are shown for the two vaccinated groups. Each mouse antibody titer was determined by endpoint dilution and is represented by a data point. The y-axis represents the serum fold dilution, and the x-axis represents the treatment groups. The dashed horizontal line represents the cut-off, which is the average OD of CpG sera + 5 x SD.