GM-CSF orchestrates monocyte and granulocyte responses to Cryptococcus gattii
Fig 5
GM-CSF induces the pulmonary influx of eosinophils over neutrophils during infection.
(A-B) Representative H&E-stained lung sections from WT (A) and Csf2-/- (B) mice at Day 10 p.i. showing perivascular cell infiltrates, scale bar = 25 μM at 20X magnification. Black boxes are magnified in the bottom panels, scale bar = 12 μM at 60X magnification. BV (Blood vessel). Histology images are representative of n = 4-5 total mice per group from N = 1 experiment; all fields of one lung slice from each mouse were evaluated. (C-E) Flow cytometry of lung cells from WT (white circles) and Csf2-/- (blue circles) mice, including eosinophils (C) neutrophils (D) and the calculated eosinophil-to-neutrophil (Eos:Neu) cellular ratio (E); a ratio of 1:1 is indicated by the dotted line. Results are from n = 7-9 total mice per group at Day 5 p.i. from N = 2 independent experiments; and n = 16 total mice per group at Day 10 p.i. from N = 4 independent experiments. (F-J) Pulmonary levels of cytokines IL-5 (F), G-CSF (G), IL-12p40 (H), IL-12p70 (I), and IL-17 (J). Naive results are from n = 5 total mice per group from N = 1 experiment; Day 5 results are from n = 3-4 total mice per group from N = 1 experiment; and Day 10 results are from n = 8-10 total mice per group from N = 2 independent experiments. Data are presented as mean ± SEM and analyzed using two-way ANOVA (C-J). *, P < 0.05. **, P < 0.01. ***, P < 0.001. ****, P < 0.0001.