A non-classical PUF family protein in oomycetes functions as a pre-rRNA processing regulator and a target for RNAi-based disease control
Fig 5
Genes associated with ribosome biogenesis are bound and regulated by PuPuf4.
(A) Sequence of the enriched motif among the peak sequences of PuPuf4 target genes. (B) In vitro binding of PuPuf4 to three motifs in the EMSA assay. (C) EMSA assay showing that non-canonical PUF proteins including ScPuf6 and APUM24 bound AGAAGAA. (D) Principal component analysis of transcriptome data and overview of DEGs in the PuPuf4 knockout compared to WT. (E) GO enrichment results of DEGs between WT and ΔPuPuf4. (F) Distribution of PuPuf4-bound peaks within protein-coding gene bodies divided into 5′-untranslated regions (UTRs), coding sequences (CDSs), 3′-UTRs, and intronic regions. Venn diagram showing the overlap of DEGs and motif-related genes with GO enrichment results of the overlapping genes.