SARS-CoV-2-induced cytokine storm drives prolonged testicular injury and functional impairment in mice that are mitigated by dexamethasone
Fig 5
SARS-CoV-2 infection leads to a transient increase in the infiltration of CD68+ and CD11b+ cells in the testis.
Representative images of testis sections at indicated days after infection (D). The DAPI (blue) and infiltrating leukocytes were visualized using (A) CD68, and (B) CD11b markers. Dotted lines outline the shape of individual seminiferous tubules and ImageJ analysis of CD68 and CD11b staining normalized to DAPI-positive cells was done using particle analyzer tool. Each data point is the average of counts from 3 different fields from each testis section (n, 3 mice/time point). Images were taken using Leica SP3, DMI8 confocal microscope. Significance (*p<0.05, **p<0.01, ***p<0.001) was determined using the student’s t-test.