Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS)
Fig 5
NMR analysis of uncleaved and cleaved VPS.
(A) Superposition of 1D 13C NMR spectra collected on uncleaved VPS (using CPMAS solid-state NMR spinning at 10,000 Hz, blue) and RbmB-cleaved VPS (solution NMR, red) shows the presence of new peaks in the cleaved sample (carbons numbered as shown on proposed modified gulose structure). The chemical shifts of these two new peaks are consistent with formation of alkene carbons predicted to result from lyase activity on the gulose moiety. The inset table shows chemical shift values (in ppm) for uncleaved VPS as reported in [21] and cleaved VPS from the solution NMR data. (B) Solution 2D [13C, 13C] COSY experiment run on RbmB-cleaved VPS shows assignments for carbons around the gulose ring. Green dotted lines illustrate the connectivity of carbon atoms around the gulose ring as labeled in (A).